The scientific value of isolated human populations is important for the understanding of ethnic formation, or for micro-evolution. The population of Sarakatsani in HELLAS was nomadic up to 10–15 years ago. It may still be considered as nomadic since it migrates during winter and summer. We studied three Sarakatsanic groups, geographically isolated in Epirus, Central Macedonia and Peloponessos. Nine cephalometric variables were measured and subjected to Discriminant Function Analysis (DFA) and Analysis of Variance (ANOVA). The latter showed that 55% of the variables indicate a statistically significant difference. The former showed that only the Peloponesean population is well differentiated. The groups from Epirus and Macedonia differentiated between them less, compared to the Peloponesean group.