The prospect for establishing a General Clinic at the Thessaly Region was examined. The new facility aimes to provide full medical care by qualified scientists (permanent personnel, shareholders or associates), by experienced, trained and skilled nursing personnel, fully organised with sophisticated technological equipment, in a hospitable and pleasant environment, with easy and fast access. The main aim of this study is the determination of the optimum location for the construction of the proposed medical centre for providing high level services in the Thessaly region, by implementing quantitative methods of Spatial Analysis of GIS technology. Spatial Analysis focuses on evaluating existing and proposed models of spatial organization, while GIS, provide the necessary informational tools for the elaboration and management of the relevant information and all in all in the support of the relevant decision making process.