High Resolution NMR spectroscopy offers a fast and sensitive method for qualitative and molecular structure analysis of polar lipids (PhL) and their main compounds (PC and PE). Lipids of marine shrimp Penaeus kerathurus were extracted according to the method of Bligh and Dyer. SPE technique was implemented to separate polar from total lipids. High resolution NMR spectroscopy has been applied for the assessment of lipid classes in marine phospholipids. More specifically, 1D 1H and 2D COSY and HSQC techniques were implemented in order to elucidate the characteristic peaks of phospholipids in the polar extracts. Furthermore, 31P NMR and spiking experiments using reference standard polar lipids (PC, PE, SPH, LPC) differentiated phospholipids and assigned several peaks to specific lipid classes. In conclusion, marine shrimp Penaeus kerathurus contains in high concentration PC and PE phospholipids and less SPH and LPC. Low inensity deshielded peaks (around 7-8 ppm) were attributed to unknown compounds for further investigations. Furthermore, carotenoid pigments are responsible for the more or less marked colouration of marine shrimps. The carotenoid profile and content of Penaeus kerathurus muscle and cephalothorax lipids, were evaluated using high-performance liquid chromatography (HPLC). The major carotenoids found were astaxanthin as well as its mono- and di- esters.