The spatial and temporal variability of the Atlantic Water (AW) and the mid-Mediterranean jet (MMJ) in the Eastern Mediterranean Levantine basin is investigated using in-situ data sets collected between 1995 and 2005. The data provide insight on the mesoscale spatial variability, the seasonal and even the hourly variability of the AW in the area of interest. Synoptic and high frequency in-situ data from hydrological surveys and an open sea observatory indicate the MMJ meanders eastward, between the southern shore of Cyprus and the northern periphery of the Cyprus warm core eddy or the Shikmona gyre. The MMJ is documented to transfer eastward the AW, both at surface and subsurface layers. The results confirm that the MMJ, as an offshore cross-basin jet, is indeed the major driving force responsible for the eastward spreading of the main volume of the AW in the SE Levantine basin, while in-situ data closer to Egypt provide evidence of a westward recirculation offshore Egypt.