The effect of neonatal jaundice on biotinidase activity

dc.contributor.author	Σούλπη, Κλεοπάτρα	el
dc.contributor.author	Γαβρίλη, Σταυρούλα	el
dc.contributor.author	Τζαμουράνη, Ιωάννα	el
dc.contributor.author	Καρίκας, Γεώργιος-Αλβέρτος	el
dc.contributor.author	Καπίκη, Αγγελική	el
dc.date.accessioned	2015-05-08T22:43:06Z
dc.date.available	2015-05-08T22:43:06Z
dc.date.issued	2015-05-09
dc.identifier.uri	http://hdl.handle.net/11400/10017
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S037837820200097X	en
dc.subject	Ίκτερος
dc.subject	Προληπτικός έλεγχος των νεογνών
dc.subject	Χολερυθρίνη
dc.subject	Βιοτινιδάσης
dc.subject	Jaundice
dc.subject	Neonatal screening
dc.subject	Bilirubin
dc.subject	Biotinidase
dc.title	The effect of neonatal jaundice on biotinidase activity	en
heal.type	journalArticle
heal.classification	Ανθρώπινη φυσιολογία
heal.classification	Νεογνά
heal.classification	Human physiology
heal.classification	Newborn infants
heal.classificationURI	N/A-Ανθρώπινη φυσιολογία
heal.classificationURI	N/A-Νεογνά
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85062884
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh2010011923
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85069783
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85014033
heal.contributorName	Κώσταλος, Χρήστος	el
heal.identifier.secondary	DOI: 10.1016/S0378-3782(02)00097-X
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2003-05
heal.bibliographicCitation	Schulpis, K.H., Gavrili, S., Tjamouranis, J., Karikas, G.A., Kapiki, A. et al. (2003) The effect of neonatal jaundice on biotinidase activity. Early Human Development. [Online] 72 (1). pp.15-24. Available from: http://www.sciencedirect.com [Accessed 08/05/2015]	en
heal.abstract	Background: Jaundice is one of the most common and one of the vexing problems that can occur in newborns. A newborn screening test for biotinidase deficiency has been added to many national screening programmes. Aim: To clarify the problem of false-positive screening tests in neonates, especially in term babies, we evaluated the biotinidase activity in the serum of fullterm, premature and small-for-dates newborn infants with jaundice. Methods: 1296 fullterms (controls N=426), 246 prematures (controls N=86) and 156 small-for-dates babies (controls N=38) aged 2–3 days with jaundice were included in the study. In jaundiced neonates and controls, 3.0 ml of blood was drawn for the evaluation of total bilirubin (t.bil), liver enzymes and biotinidase activity in the serum using a fluorimetric method. In order to test whether or not t.bil causes an artifact in the previous method, biotinidase activity was also evaluated in a number of jaundiced newborns using an HPLC method. Additionally, a preliminary in vitro experiment was carried out to test whether t.bil is an inhibitor of the enzyme. Results: Biotinidase activities in the group of controls of prematures (3.30±1.2 mmol/min/l) and small-for-dates babies (3.34±0.8 mmol/min/l) were lower than those of term babies (4.99±1.1 mmol/min/l, p<0.001). T.bil and liver enzymes showed a statistically significant inverse correlation with biotinidase activity (p<0.001) in all the jaundiced infants of this study. Additionally, biotinidase activity, evaluated in a number of neonates with both fluorimetric and HPLC methods showed similar results. Preincubation of the serum enzyme with t.bil (>10 mg/dl) resulted in a 50% or more inhibition. Conclusions: (a) Low biotinidase activity was found in term babies, prematures and small-for-dates with jaundice. (b) The low activity of the enzyme could be due to their impaired liver function. (c) The high t.bil levels in the studied groups may play the role of an “inhibitor” of the enzyme. (d) Gestational age as well as t.bil levels should always be written on Guthrie cards for a correct evaluation of biotinidase activity.	en
heal.publisher	Elsevier	en
heal.journalName	Early Human Development	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true