In vivo effects of high phenylalanine blood levels on Na+,K+-ATPase, Mg2+-ATPase activities and biogenic amine concentrations in phenylketonuria

dc.contributor.author	Σούλπη, Κλεοπάτρα	en
dc.contributor.author	Τζαμουράνη, Ιωάννα	el
dc.contributor.author	Καρίκας, Γεώργιος-Αλβέρτος	el
dc.contributor.author	Μιχελακάκη, Ελένη	el
dc.contributor.author	Τσακίρης, Στυλιανός	el
dc.date.accessioned	2015-05-09T17:49:19Z
dc.date.available	2015-05-09T17:49:19Z
dc.date.issued	2015-05-09
dc.identifier.uri	http://hdl.handle.net/11400/10033
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.ncbi.nlm.nih.gov/pubmed/12135689?dopt=AbstractPlus	en
dc.subject	Βιογενείς αμίνες
dc.subject	επίπεδα της φαινυλαλανίνης στο αίμα
dc.subject	φαινυλκετονουρία
dc.subject	αμινοξέα
dc.subject	Μεμβράνες ερυθροκυττάρων
dc.subject	Biogenic amines
dc.subject	Phenylalanine blood levels
dc.subject	Phenylketonuria
dc.subject	Amino acids
dc.subject	Erythrocyte membranes
dc.title	In vivo effects of high phenylalanine blood levels on Na+,K+-ATPase, Mg2+-ATPase activities and biogenic amine concentrations in phenylketonuria	en
heal.type	journalArticle
heal.classification	Κλινική βιοχημεία
heal.classification	Διατροφή
heal.classification	Clinical biochemistry
heal.classification	Nutrition
heal.classificationURI	N/A-Κλινική βιοχημεία
heal.classificationURI	N/A-Διατροφή
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85027062
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85093451
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85014143
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85100698
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85004486
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh89006625
heal.identifier.secondary	DOI: 10.1016/S0009-9120(02)00311-9
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2002-06
heal.bibliographicCitation	Schulpis, K.H., Tjamouranis, J., Karikas, G.A., Michelakakis, H. and Tsakiris, S. (2002) In vivo effects of high phenylalanine blood levels on Na+,K+-ATPase, Mg2+-ATPase activities and biogenic amine concentrations in phenylketonuria. Clinical Biochemistry. [Online] 35 (4). pp.281-285. Available from: http://www.ncbi.nlm.nih.gov [Accessed 09/05/2015]	en
heal.abstract	Objective: To evaluate the activities of Na+,K+-ATPase and Mg2+-ATPase in erythrocyte membranes from phenylketonuric (PKU) patients and to correlate the enzyme activities with their blood phenylalanine (Phe) levels, biogenic amines as well as with their precursors tyrosine (Tyr) and tryptophan (Try). Design and methods: Twenty three PKU patients were divided into group A (n = 12) on a stricted diet (Phe 1.57 ± 0.52 mg/dL or 0.10 ± 0.03 mM) and group B (n = 11) on a “loose” diet (Phe 24.45 ± 1.50 mg/dL or 1.72 ± 0.09 mM). The enzyme activities were measured spectrophotometrically, the amino acids with an automatic amino analyser and the biogenic amines with HPLC methods. Results: In group B, plasma amino acids (Tyr, Try), their biogenic amines [adrenaline (A), noradrenaline (NA), dopamine (DA) and serotonin (5HT)], (Na+,K+)-ATPase and Mg2+-ATPase activities were found remarkably decreased (p < 0.001). Conclusions: High Phe and/or low NA, DA, 5HT plasma levels may indirectly inhibit the erythrocyte membrane Na+,K+-ATPase and Mg2+-ATPase in PKU patients. The observed enzyme inhibitions could be a very informative peripheral marker as regards the neurotoxic Phe brain effects.	en
heal.publisher	Elsevier	en
heal.journalName	Clinical Biochemistry	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true