Reduced acetylcholinesterase activity in erythrocyte membranes from patients with phenylketonuria

dc.contributor.author	Τσακίρης, Στυλιανός	el
dc.contributor.author	Σούλπη, Κλεοπάτρα	el
dc.contributor.author	Τζαμουράνη, Ιωάννα	el
dc.contributor.author	Μιχελακάκη, Ελένη	el
dc.contributor.author	Καρίκας, Γεώργιος-Αλβέρτος	el
dc.date.accessioned	2015-05-09T18:02:06Z
dc.date.available	2015-05-09T18:02:06Z
dc.date.issued	2015-05-09
dc.identifier.uri	http://hdl.handle.net/11400/10036
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.ncbi.nlm.nih.gov/pubmed/12498995?dopt=AbstractPlus	en
dc.subject	Δραστικότητας της AChE
dc.subject	φαινυλαλανίνη
dc.subject	τυροσίνη
dc.subject	ντοπαμίνη
dc.subject	αλανίνη
dc.subject	AChE activity
dc.subject	phenylalanine
dc.subject	Tyrosine
dc.subject	Dopamine
dc.subject	Alanine
dc.title	Reduced acetylcholinesterase activity in erythrocyte membranes from patients with phenylketonuria	en
heal.type	journalArticle
heal.classification	Κλινική βιοχημεία
heal.classification	Ανθρώπινη φυσιολογία
heal.classification	Clinical biochemistry
heal.classification	Human physiology
heal.classificationURI	N/A-Κλινική βιοχημεία
heal.classificationURI	N/A-Ανθρώπινη φυσιολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85027062
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85062884
heal.keywordURI	http://lod.nal.usda.gov/7802
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85139195
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85039075
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85003136
heal.identifier.secondary	DOI: 10.1016/S0009-9120(02)00381-8
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2002-11
heal.bibliographicCitation	Tsakiris, S., Schulpis, K.H., Tjamouranis, J., Michelakakis, H., and Karikas, G.A. (2002) Reduced acetylcholinesterase activity in erythrocyte membranes from patients with phenylketonuria. Clinical Biochemistry. [Online] 35 (8). pp.615-619. Available from: http://www.ncbi.nlm.nih.gov [Accessed 09/05/2015]	en
heal.abstract	Objective: a) To evaluate acetylcholinesterase (AChE) activities in erythrocyte membranes from phenylketonuric (PKU) patients and controls and to correlate with their plasma phenylalanine (Phe), tyrosine (Tyr), alanine (Ala) and dopamine (DA) levels. b) To determine the in vitro effects of Phe, Ala and Phe plus Ala on their AChE activities. Design and Methods: AChE activities were determined spectrophotometrically in erythrocyte membranes from PKU children (n = 12) adhering to their diet (group A), from 11 “off diet” (group B) and from 23 controls. Their plasma amino acids were evaluated with an amino acid analyser and DA with an HPLC method. Ala (1.8 mM) and/or Phe (1.8 mM) were added in the enzyme incubation medium from controls, whereas only Ala was added in that from group B. Results: Tyr (46 ± 17 μmol/L) and DA (56 ± 18 μmol/L) were remarkably decreased by about 60% in group B as compared to those of group A (respectively, p < 0.001) and controls (respectively, p < 0.001). Phe negatively correlated with AChE activity and positively with plasma Tyr and DA. Ala reversed the inhibited AChE by Phe in erythrocyte membranes from healthy children to control values, whereas no reverse effect was observed on the enzyme activity from PKU patients. Conclusions: a) The low levels of DA and its precursor Tyr are due to the high Phe blood levels, as a consequence of the decreased activity of Phe-hydroxylase in the liver of our patients. So, high Phe blood levels inhibit AChE in PKU patients, probably resulting in higher acetylcholine concentrations. b) Determination of AChE in erythrocyte membranes from PKU could be a useful marker for the neurotoxic effects of Phe.	en
heal.publisher	Elsevier	en
heal.journalName	Clinical Biochemistry	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true