Extraction-less, rapid assay for the direct detection of 2,4,6-trichloroanisole (TCA) in cork samples

dc.contributor.author	Αποστόλου, Θεοφύλακτος	el
dc.contributor.author	Pascual, Núria	en
dc.contributor.author	Marco, Maria-Pilar	en
dc.contributor.author	Μόσχος, Αναστάσιος	el
dc.contributor.author	Πετρόπουλος, Αναστάσιος	el
dc.date.accessioned	2015-05-26T06:19:25Z
dc.date.available	2015-05-26T06:19:25Z
dc.date.issued	2015-05-26
dc.identifier.uri	http://hdl.handle.net/11400/11160
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.elsevier.com/	en
dc.subject	2,4,6-trichloroanisole (TCA)
dc.subject	Bioelectric Recognition Assay (BERA)
dc.subject	Cellular biosensor
dc.subject	Cork taint
dc.subject	Membrane-engineering
dc.subject	2,4,6-Τριχλωροανισόλη
dc.subject	Κινητός βιοαισθητήρας
dc.subject	Μεμβράνη μηχανικής
dc.subject	Προσδιορισμός βιοηλεκτρικής αναγνώρισης
dc.title	Extraction-less, rapid assay for the direct detection of 2,4,6-trichloroanisole (TCA) in cork samples	en
heal.type	journalArticle
heal.classification	Technology
heal.classification	Electrical engineering
heal.classification	Τεχνολογία
heal.classification	Ηλεκτρολογία Μηχανολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	http://zbw.eu/stw/descriptor/18426-4
heal.classificationURI	N/A-Τεχνολογία
heal.classificationURI	N/A-Ηλεκτρολογία Μηχανολογία
heal.contributorName	Καλτσάς, Γρηγόριος	el
heal.contributorName	Κίντζιος, Σπυρίδων	el
heal.identifier.secondary	DOI: 10.1016/j.talanta.2014.03.023
heal.language	en
heal.access	campus
heal.publicationDate	2014-07-01
heal.bibliographicCitation	APOSTOLOU, T., PASCUAL, N., MARCO, M.-P., MOSCHOS, A., PETROPOULOS, A., et al. (2014). Extraction-less, rapid assay for the direct detection of 2,4,6-trichloroanisole (TCA) in cork samples. Talanta. [online] 125. p. 336-340. Available from: http://www.elsevier.com/[Accessed 20/03/2014]	en
heal.abstract	2,4,6-trichloroanisole (TCA), the cork taint molecule, has been the target of several analytical approaches over the few past years. In spite of the development of highly efficient and sensitive tools for its detection, ranging from advanced chromatography to biosensor-based techniques, a practical breakthrough for routine cork screening purposes has not yet been realized, in part due to the requirement of a lengthy extraction of TCA in organic solvents, mostly 12% ethanol and the high detectability required. In the present report, we present a modification of a previously reported biosensor system based on the measurement of the electric response of cultured fibroblast cells membrane-engineered with the pAb78 TCA-specific antibody. Samples were prepared by macerating cork tissue and mixing it directly with the cellular biorecognition elements, without any intervening extraction process. By using this novel approach, we were able to detect TCA in just five minutes at extremely low concentrations (down to 0.2 ppt). The novel biosensor offers a number of practical benefits, including a very considerable reduction in the total assay time by one day, and a full portability, enabling its direct employment for on-site, high throughput screening of cork in the field and production facilities, without requiring any type of supporting infrastructure.	en
heal.publisher	Elsevier	en
heal.journalName	Talanta	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true