High throughput cellular biosensor for the ultra-sensitive, ultra-rapid detection of aflatoxin M1

dc.contributor.author	Λάρου, Ευαγγελία	el
dc.contributor.author	Γιακουμεττής, Ιάκωβος	el
dc.contributor.author	Καλτσάς, Γρηγόριος	el
dc.contributor.author	Πετρόπουλος, Αναστάσιος	el
dc.contributor.author	Σκανδάμης, Παναγιώτης Ν.	el
dc.date.accessioned	2015-05-26T06:51:56Z
dc.date.available	2015-05-26T06:51:56Z
dc.date.issued	2015-05-26
dc.identifier.uri	http://hdl.handle.net/11400/11163
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.elsevier.com/	en
dc.subject	Aflatoxin M1 (AFM1)
dc.subject	Bioelectric recognition assay
dc.subject	Cell biosensor
dc.subject	Membrane-engineering
dc.subject	Αφλατοξίνη Μ1
dc.subject	Προσδιορισμός βιοηλεκτρικής αναγνώρισης
dc.subject	Βιοαισθητήρας κυττάρων
dc.subject	Μεμβράνη μηχανικής
dc.title	High throughput cellular biosensor for the ultra-sensitive, ultra-rapid detection of aflatoxin M1	en
heal.type	journalArticle
heal.classification	Technology
heal.classification	Chemical technology
heal.classification	Τεχνολογία
heal.classification	Χημική τεχνολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	http://skos.um.es/unescothes/C00565
heal.classificationURI	N/A-Τεχνολογία
heal.classificationURI	N/A-Χημική τεχνολογία
heal.contributorName	Κίντζιος, Σπυρίδων	el
heal.identifier.secondary	DOI: 10.1016/j.foodcont.2012.06.012
heal.language	en
heal.access	campus
heal.publicationDate	2013-01
heal.bibliographicCitation	LAROU, E., YIAKOUMETTIS, I., KALTSAS, G., PETROPOULOS, A., SKANDAMIS, P.N., et al. (2013). High throughput cellular biosensor for the ultra-sensitive, ultra-rapid detection of aflatoxin M1. Food Control. [online] 29 (1). p. 208-212. Available from: http://www.elsevier.com/[Accessed 18/06/2012]	en
heal.abstract	The aim of this study was the development of a rapid novel biosensor system based on the Bioelectric Recognition Assay (BERA) for the detection of aflatoxin M1 (AFM1). Membrane-engineering was achieved by electroinsertion of AFM1-homologous antibodies into the membrane of the cells. The sensor measured the electric response of cultured membrane-engineered fibroblast cells suspended in an alginate gel matrix due to the change of their membrane potential after the interaction of the analyte molecules with the antibodies. The BERA-based sensor was able to detect AFM1 rapidly (3min) at very low concentrations (5 pg/ml = 5 ppt), thus demonstrating a higher sensitivity than most currently available biosensor-based methods. In addition, the assay was quite selective against other aflatoxins, such as aflatoxin B1 and ochratoxin A. Furthermore, the assay system allowed for high throughput AFM1 analysis (160 individual tests/h), due to its multiple cell-electrode interface array. Although further research is required for the optimization of the assay, the elimination of possible matrix effects and its validation by assaying actual food samples, this novel biosensor offers new perspectives for ultra-rapid, ultra-sensitive and low-cost monitoring of mycotoxins in food commodities.	en
heal.publisher	Elsevier	en
heal.journalName	Food Control	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true