Direct measurement of CD34+ cord blood stem cell absolute counts, viability and WBC using flow cytometry

dc.contributor.author	Καλοδήμου, Βασιλική Ε.	el
dc.contributor.author	Μπίκος, Λευτέρης	el
dc.contributor.author	Κουρεντή, Καλλιόπη	el
dc.contributor.author	Φίφα, Αικατερίνη	el
dc.contributor.author	Βλαχούσης, Αντώνιος	el
dc.date.accessioned	2014-07-15T12:43:39Z
dc.date.available	2014-07-15T12:43:39Z
dc.date.issued	2014-07-15
dc.identifier.uri	http://hdl.handle.net/11400/1320
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	www.hsj.gr	en
dc.subject	Flow cytometry
dc.subject	Neural stem cells
dc.subject	Fetal blood
dc.subject	Leucocytes
dc.subject	Viability (Biology)
dc.subject	CD34 antigen
dc.title	Direct measurement of CD34+ cord blood stem cell absolute counts, viability and WBC using flow cytometry	en
heal.type	journalArticle
heal.classification	Medicine
heal.classification	Ιατρική
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh00006614
heal.classificationURI	N/A-Ιατρική
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85049287
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh2003001310
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85047943
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85076269
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh2012001925
heal.language	en
heal.access	free
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας.Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Νοσηλευτικής	el
heal.publicationDate	2011-12
heal.bibliographicCitation	Kalodimou, V.E., Bikos, L., Kourenti, C., Fifa, A., Vlachousis, A.. (2011). Direct measurement of CD34+ cord blood stem cell absolute counts, viability and WBC using flow cytometry. Health Science Journal". 5 (4), 306-19.	en
heal.abstract	Background: flow cytometry is a technique of quantitative single cell analysis. It can process measurements on thousands cells in a few seconds. Since different cell types can be distinguished by quantization structural features, flow cytometry can be used to count cells of different types in a mixture. Cord blood samples, has posed additional challenges over the years particularly due to the presence of varying number of nucleated red blood cells (RBC) and the extended age of the samples at the time of CD34+ cells enumeration. Method: the aim in our study was to prove that time has no effect in CD34+ cells storage, efficacy and viability using flow cytometry as a quantitative single cell analysis. To enhance further our study, post-freeze white blood cells (WBC) counts from an automated haematology analyzer were compared with WBC derived after cytometric analysis. Results: four-thousands cord blood samples were analyzed for absolute viable CD34+ cells, WBC and their viability. Viable CD34+ cells were enumerated using single platform flow cytometry and the molecular exclusion dye 7-amino actinomycin D (7- AAD). Conclusion: cryopreservation and processing of autologous stem cells collection, assessing CD34+ cell viability and absolute counts, found to remain the same during periods of time. Measuring CD34 +cell viability allows us the quality of control assessments of stem cells processing.	en
heal.publisher	Μαρβάκη, Χριστίνα	el
heal.journalName	Health Science Journal	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true
heal.dateCreated	2011-10