Highly versatile confocal microscopy system based on a tunable femtosecond Er: Fiber source

dc.contributor.author	Träutlein, Daniel	en
dc.contributor.author	Adler, Florian	en
dc.contributor.author	Μουτζούρης, Κωνσταντίνος Ι.	el
dc.contributor.author	Jeromin, Andreas	en
dc.contributor.author	Leitenstorfer, Alfred	en
dc.date.accessioned	2015-06-04T18:01:58Z
dc.date.issued	2015-06-04
dc.identifier.uri	http://hdl.handle.net/11400/15111
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.wiley-vch.de/	en
dc.subject	DNA damage
dc.subject	Femtosecond technology
dc.subject	Fluorescent protein
dc.subject	Multiphoton microscopy
dc.subject	Photomanipulation
dc.subject	Three-photon absorption
dc.subject	Βλάβη του DNA
dc.subject	Φθορίζουσα πρωτεΐνη
dc.subject	Πολυφωτονικό μικροσκόπιο
dc.subject	Απορρόφηση τριών φωτονίων
dc.title	Highly versatile confocal microscopy system based on a tunable femtosecond Er: Fiber source	en
heal.type	journalArticle
heal.classification	Technology
heal.classification	Electrical engineering
heal.classification	Τεχνολογία
heal.classification	Ηλεκτρολογία Μηχανολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	http://zbw.eu/stw/descriptor/18426-4
heal.classificationURI	N/A-Τεχνολογία
heal.classificationURI	N/A-Ηλεκτρολογία Μηχανολογία
heal.contributorName	Ferrando-May, Elisa	en
heal.identifier.secondary	DOI: 10.1002/jbio.200710019
heal.dateAvailable	10000-01-01
heal.language	en
heal.access	forever
heal.publicationDate	2008
heal.bibliographicCitation	TRAUTLEIN, D., ADLER, F., MOUTZOURIS, K.I., JEROMIN, A., LEITENSTORFER, A., et al. (2008). Highly versatile confocal microscopy system based on a tunable femtosecond Er: Fiber source. Journal of Biophotonics. [online] 1 (1). p. 53-61. Available from: http://www.wiley-vch.de/[Accessed 08/01/2008]	en
heal.abstract	The performance of a confocal microscopy setup based on a single femtosecond fiber system is explored over a broad range of pump wavelengths for both linear and nonlinear imaging techniques. First, the benefits of a laser source in linear fluorescence excitation that is continuously tunable over most of the visible spectrum are demonstrated. The influences of subpicosecond pulse durations on the bleaching behavior of typical fluorophores are discussed. We then utilize the tunable near-infrared output of the femtosecond system in connection with a specially designed prism compressor for dispersion control. Pulses as short as 33 fs are measured in the confocal region. As a consequence, 2 mW of average power are sufficient for twophoton microscopy in an organotypic sample from the mouse brain. This result shows great prospect for deep-tissue imaging in the optimum transparency window around 1100 nm. In a third experiment, we prove that our compact setup is powerful enough to exploit even higher-order nonlinearities such as three-photon absorption that we use to induce spatially localized photodamage in DNA.	en
heal.publisher	Wiley-VCH Verlag	en
heal.journalName	Journal of Biophotonics	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false