Impact of Toll-like receptor-4 and tumour necrosis factor gene polymorphisms in patients with hidradenitis suppurativa

dc.contributor.author	Σάββα, Αθηνά	el
dc.contributor.author	Κάννη, Θεοδώρα	el
dc.contributor.author	Δαμοράκη, Γεωργία	el
dc.contributor.author	Κωτσάκη, Αντιγόνη	el
dc.contributor.author	Γιατράκου, Σοφία	el
dc.date.accessioned	2015-06-07T19:54:57Z
dc.date.available	2015-06-07T19:54:57Z
dc.date.issued	2015-06-07
dc.identifier.uri	http://hdl.handle.net/11400/15541
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://onlinelibrary.wiley.com	en
dc.subject	Ογκολογία
dc.subject	DNA
dc.subject	Oncology
dc.subject	Tumor necrosis
dc.subject	Νέκρωση όγκου
dc.title	Impact of Toll-like receptor-4 and tumour necrosis factor gene polymorphisms in patients with hidradenitis suppurativa	en
heal.type	journalArticle
heal.classification	Aesthetics
heal.classification	Cosmetics
heal.classification	Αισθητική
heal.classification	Κοσμητολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85001441
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85033139
heal.classificationURI	N/A-Αισθητική
heal.classificationURI	N/A-Κοσμητολογία
heal.contributorName	Γκρεκ, Ιωάννα	el
heal.contributorName	Κατούλης, Αλέξανδρος	el
heal.contributorName	Παπαδαυίδ, Ευαγγελία	el
heal.contributorName	Γιαμαρέλλος - Μπουρμπούλης, Ευάγγελος	el
heal.identifier.secondary	DOI: 10.1111/bjd.12105
heal.language	en
heal.access	campus
heal.publicationDate	2013
heal.bibliographicCitation	Savva, A., Kanni, T., Damoraki, G., Kotsaki, A., Giatrakou, S. et al. (2013) Impact of Toll-like receptor-4 and tumour necrosis factor gene polymorphisms in patients with hidradenitis suppurativa. "British Journal of Dermatology", 168 (2), p.311-317	en
heal.abstract	Background Recent evidence has suggested that deranged immune responses play a role in the pathogenesis of hidradenitis suppurativa (HS). Objectives To investigate the role of single nucleotide polymorphisms (SNPs) of the tumour necrosis factor (TNF) and Toll-like receptor 4 (TLR4) genes in the physical course of HS; these genes encode for proteins implicated in the immune response of the host. Methods DNA was isolated from 190 patients with HS and 84 healthy controls. SNPs at the promoter regions -376G/A, -238G/A and -308G/A of the TNF gene and the Asp299Gly and Thr399Ile SNPs of the TLR4 gene were determined by polymerase chain reaction (PCR) and digestion of the PCR product by restriction enzymes; after electrophoresis on 2·0% agarose gel, products were visualized on under ultraviolet radiation. Results The presence of the -238 TNF gene polymorphism was associated with a predisposition to HS (P = 0·027). Susceptibility to the disease was strongly correlated with the presence of AGG/GGA/AGA/GAA TNF haplotypes in 32 (17%) patients compared with two (2%) controls (P < 0·001, odds ratio 8·30, 95% confidence interval 1·94-35·52). The frequency of HS exacerbations and disease severity were greater in patients carrying any of the GAG/AGG/GGA/AGA/GAA haplotypes of the TNF gene. Thirty-two patients were given TNF antagonists. Nineteen of these patients were carriers of the GGG haplotype of the TNF gene, whereas 13 were carriers of other haplotypes; favourable responses as evidenced by the Sartorius score were registered in 15 (79%) and five (38%, P = 0·025), respectively. Carriage of the TLR4 gene alleles was not associated with any disease parameter. Conclusions A significant role of SNPs at the promoter region of the TNF gene is indicated for susceptibility to HS and for response to TNF antagonists. © 2012 The Authors.	en
heal.journalName	British Journal of Dermatology	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true