Comparative in vitro stability and scintigraphic imaging for trafficking and tumor targeting of a directly and a novel 99mTc (I)(CO)3 labeled liposome

dc.contributor.author	Φραγκογιώργη, Ειρήνη Α.	el
dc.contributor.author	Savina, Irina N.	en
dc.contributor.author	Τσοτάκος, Θεόδωρος	el
dc.contributor.author	Ευθυμίου, Ελένη Κ.	el
dc.contributor.author	Ξανθόπουλος, Σταύρος	el
dc.date.accessioned	2015-06-10T15:11:15Z
dc.date.available	2015-06-10T15:11:15Z
dc.date.issued	2015-06-10
dc.identifier.uri	http://hdl.handle.net/11400/15642
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S0378517314000817	en
dc.subject	Άμεση επισήμανση
dc.subject	παθητική στόχευση
dc.subject	Πολυαιθυλενογλυκολιωμένες λιποσωμάτων
dc.subject	Σπινθηρογραφική απεικόνιση
dc.subject	Επιφάνεια επισήμανσης αποσιδήρωσης
dc.subject	Direct labeling
dc.subject	Passive targeting
dc.subject	Pegylated liposome
dc.subject	Scintigraphic imaging
dc.subject	Surface chelation labeling
dc.title	Comparative in vitro stability and scintigraphic imaging for trafficking and tumor targeting of a directly and a novel 99mTc (I)(CO)3 labeled liposome	en
heal.type	journalArticle
heal.classification	Χημεία
heal.classification	Φαρμακευτική
heal.classification	Chemistry
heal.classification	Pharmacy
heal.classificationURI	N/A-Χημεία
heal.classificationURI	N/A-Φαρμακευτική
heal.classificationURI	http://skos.um.es/unescothes/C00568
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85100603
heal.contributorName	Παλαμάρης, Λάζαρος	el
heal.contributorName	Ψιμάδας, Δημήτριος	el
heal.contributorName	Μπουζιώτη, Πηνελόπη	el
heal.contributorName	Κόρδας, Γεώργιος	el
heal.contributorName	Mikhalovsky, Sergey	en
heal.contributorName	Alavijeh, Mohammad	en
heal.contributorName	Λούντος, Γεώργιος	el
heal.identifier.secondary	DOI: 10.1016/j.ijpharm.2014.01.042
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογικών Εφαρμογών. Τμήμα Μηχανικών Βιοϊατρικής Τεχνολογίας Τ.Ε.	el
heal.publicationDate	2014-04-25
heal.bibliographicCitation	Fragogeorgi, E.A., Savina, I.N., Tsotakos, T., Efthimiadou, E., Xanthopoulos, S. et al. (2014) Comparative in vitro stability and scintigraphic imaging for trafficking and tumor targeting of a directly and a novel 99mTc (I)(CO)3 labeled liposome. International Journal of Pharmaceutics. [Online] 465 (1-2), pp.333-346. Available from: http://www.sciencedirect.com [Accessed 10/06/2015]	en
heal.abstract	Liposomes radiolabelling with diagnostic radionuclides is an excellent tool for studying pharmacokinetics with the view of developing liposome-based drug delivery agents. The aim of the present study is to evaluate the in vitro and in vivo behavior of a 99mTc-labeled liposome by applying either a direct labeling strategy via a carboxyl group, LP-COOH, or a surface chelating method via pyridyl ethyl cysteine compound (with the intermediate [ 99mTc(I)(CO)3(H2O)3] +), LP-PEC. 99mTc-LP-COOH was obtained in high radiolabelling yield and radiochemical purity, while 99mTc (I)(CO)3-LP-PEC was initially purified before being in vitro and in vivo evaluated. 99mTc-LP-COOH was less stable in the presence of competitive for 99mTc ligands than 99mTc (I)(CO)3-LP-PEC. According to DLS measurements, the presence of serum as well as the applied radiolabelling conditions did not affect the liposomes' size. The different radiolabelling methods seemed to exert an influence on the biodistribution pattern of the liposomes with the 99mTc(I)(CO)3-LP-PEC showing slow blood clearance, which was also confirmed by in vivo scintigraphic imaging. Nevertheless, passive tumor targeting was attained at a similar extent no matter which radiolabelling technique was followed.	en
heal.publisher	Elsevier	en
heal.journalName	International Journal of Pharmaceutics	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true