Dynamic in vivo imaging of dual-triggered microspheres for sustained release applications

dc.contributor.author	Ευθυμιάδου, Ελένη Κ.	el
dc.contributor.author	Ταπεινός, Χρήστος	el
dc.contributor.author	Χατζηπαυλίδης, Αλέξανδρος	el
dc.contributor.author	Μπούκος, Νίκος Κ.	el
dc.contributor.author	Φραγκογιώργη, Ειρήνη Α.	el
dc.date.accessioned	2015-06-10T23:19:56Z
dc.date.available	2015-06-10T23:19:56Z
dc.date.issued	2015-06-11
dc.identifier.uri	http://hdl.handle.net/11400/15667
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S0378517313010314	en
dc.subject	Βιοκατανομή
dc.subject	απελευθέρωση Dox
dc.subject	Κοίλα μικροσφαιρίδια
dc.subject	Υπερθερμία
dc.subject	Ραδιοεπισήμανση
dc.subject	Biodistribution
dc.subject	Dox release
dc.subject	Hollow microspheres
dc.subject	Hyperthermia
dc.subject	Radiolabeling
dc.title	Dynamic in vivo imaging of dual-triggered microspheres for sustained release applications	en
heal.type	journalArticle
heal.secondaryTitle	synthesis, characterization and cytotoxicity study	en
heal.classification	Φαρμακευτική
heal.classification	Καρκίνος
heal.classification	Pharmacy
heal.classification	Cancer
heal.classificationURI	N/A-Φαρμακευτική
heal.classificationURI	N/A-Καρκίνος
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85100603
heal.classificationURI	http://zbw.eu/stw/descriptor/18899-3
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh91005211
heal.contributorName	Παλαμάρης, Λάζαρος	el
heal.contributorName	Λούντος, Γεώργιος	el
heal.contributorName	Κόρδας, Γεώργιος	el
heal.identifier.secondary	DOI: 10.1016/j.ijpharm.2013.11.037
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογικών Εφαρμογών. Τμήμα Μηχανικών Βιοϊατρικής Τεχνολογίας Τ.Ε.	el
heal.publicationDate	2014-01-30
heal.bibliographicCitation	Efthimiadou, E.K., Tapeinos, C., Chatzipavlidis, A., Boukos, N., Fragogeorgi, E. et al. (2014) Dynamic in vivo imaging of dual-triggered microspheres for sustained release applications: Synthesis, characterization and cytotoxicity study. International Journal of Pharmaceutics. [Online] 461 (1-2), pp.54-63. Available from: http://www.sciencedirect.com [Accessed 10/06/2015]	en
heal.abstract	This paper deals with the synthesis, characterization and property evaluation of drug-loaded magnetic microspheres with pH-responsive cross-linked polymer shell. The synthetic procedure consists of 3 steps, of which the first two comprise the synthesis of a poly methyl methacrylate (PMMA) template and the synthesis of a shell by using acrylic acid (AA) and methyl methacrylate (MMA) as monomers, and divinyl benzene (DVB) as cross-linker. The third step of the procedure refers to the formation of magnetic nanoparticles on the microsphere's surface. AA that attaches pH-sensitivity in the microspheres and magnetic nanoparticles in the inner and the outer surface of the microspheres, enhance the efficacy of this intelligent drug delivery system (DDS), which constitutes a promising approach toward cancer therapy. A number of experimental techniques were used to characterize the resulting microspheres. In order to investigate the in vitro controlled release behavior of the synthesized microspheres, we studied the Dox release percentage under different pH conditions and under external magnetic field. Hyperthermia caused by an alternating magnetic field (AFM) is used in order to study the doxorubicin (Dox) release behavior from microspheres with pH functionality. The in vivo fate of these hybrid-microspheres was tracked by labeling them with the γ-emitting radioisotope 99mTc after being intravenously injected in normal mice. According to our results, microsphere present a pH depending and a magnetic heating, release behavior. As expected, labeled microspheres were mainly found in the mononuclear phagocyte system (MPS). The highlights of the current research are: (i) to illustrate the advantages of controlled release by combining hyperthermia and pH-sensitivity and (ii) to provide noninvasive, in vivo information on the spatiotemporal biodistribution of these microsphere by dynamic γ-imaging.	en
heal.publisher	Elsevier	en
heal.journalName	International Journal of Pharmaceutics	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true