Rapid microprapagation of grapevine CV

dc.contributor.author	Μπανίλας, Γεώργιος Π.	el
dc.contributor.author	Κόρκας, Ηλίας	el
dc.date.accessioned	2015-01-26T14:25:23Z
dc.date.available	2015-01-26T14:25:23Z
dc.date.issued	2015-01-26
dc.identifier.uri	http://hdl.handle.net/11400/4779
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://e-jst.teiath.gr/	en
dc.subject	Cytokinins
dc.subject	Κυτοκινίνες
dc.subject	Micropropagation
dc.subject	Nodal culture
dc.subject	Vitis vinifera
dc.subject	Μικροπολλαπλασιασμός
dc.subject	Κομβικός πολιτισμός
dc.title	Rapid microprapagation of grapevine CV	en
heal.type	journalArticle
heal.secondaryTitle	agiorgitiko through lateral bud development	en
heal.classification	Technology
heal.classification	Chemical technology
heal.classification	Τεχνολογία
heal.classification	Χημική τεχνολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	http://skos.um.es/unescothes/C00565
heal.classificationURI	N/A-Τεχνολογία
heal.classificationURI	N/A-Χημική τεχνολογία
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85035238
heal.language	en
heal.access	free
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών	el
heal.publicationDate	2007
heal.bibliographicCitation	Banilas, G. and Korkas, E. (2007). Rapid microprapagation of grapevine CV: agiorgitiko through lateral bud development. "e-Journal of Science & Technology". [Online] 2(3): 31-38. Available from: http://e-jst.teiath.gr/	en
heal.abstract	Micropropagation has been a well-established methodology for in vitro regeneration of grapevine (Vitis vinifera L.), usually through shoot apical meristem culture or adventitious bud formation. However, recent studies have shown that micropropagated plants often show genetic discrepancy from the mother plant, due to high levels of PGRs applied. Here we present an efficient protocol for rapid in vitro regeneration of grapevine cv. Agiorgitiko genotypes through nodal culture. Development of a single main shoot was prominent on agar-solidified basal MS medium without any PGR or supplemented with relatively low levels (e.g., up to 2.5 µM) of benzyladenine (BA). At higher BA levels axillary bud growth and shoot multiplication were enhanced, but hyperhydricity was also apparent. Relatively low concentrations of indole-3-butyric acid (IBA) promoted both the frequency of shoots forming roots and the number of roots per shoot. Although the overall yield is rather lower compared with shoot tip culture approaches, present protocol may serve as an alternative for true-to-type regeneration of selected clones of ‘Agiorgitiko’ and possibly of other grapevine cultivars.	en
heal.publisher	Νερατζής, Ηλίας	el
heal.publisher	Σιανούδης, Ιωάννης	el
heal.journalName	e-Journal of Science & Technology	en
heal.journalName	e-Περιοδικό Επιστήμης & Τεχνολογίας	el
heal.journalType	peer-reviewed
heal.fullTextAvailability	true