Taguchi experimental design for medium optimization for enhanced protease production by Bacillus subtilis HB04

dc.contributor.author	Venil, C.K.	en
dc.contributor.author	Lakshmanaperumalsamy, P.	en
dc.date.accessioned	2015-01-29T06:50:29Z
dc.date.available	2015-01-29T06:50:29Z
dc.date.issued	2015-01-29
dc.identifier.uri	http://hdl.handle.net/11400/4995
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://e-jst.teiath.gr/	en
dc.subject	Bacillus subtilis HB04
dc.subject	Protease
dc.subject	Taguchi design
dc.subject	Contribution
dc.subject	Πρωτεάση
dc.subject	Συνεισφορά
dc.subject	Taguchi σχεδιασμός
dc.title	Taguchi experimental design for medium optimization for enhanced protease production by Bacillus subtilis HB04	en
heal.type	journalArticle
heal.classification	Technology
heal.classification	Chemical technology
heal.classification	Τεχνολογία
heal.classification	Χημική τεχνολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	http://skos.um.es/unescothes/C00565
heal.classificationURI	N/A-Τεχνολογία
heal.classificationURI	N/A-Χημική τεχνολογία
heal.language	en
heal.access	free
heal.publicationDate	2009
heal.bibliographicCitation	Venil, C.K. and Lakshmanaperumalsamy, P. (2009). Taguchi experimental design for medium optimization for enhanced protease production by Bacillus subtilis HB04. "e-Journal of Science & Technology". [Online] 4(4): 1-10. Available from: http://e-jst.teiath.gr/	en
heal.abstract	Bacillus sp. is specific producers of proteases amongst bacteria and protease enzymes are of significant ones due to their multifarious applications. In this research,Taguchi experimental design was applied to optimize the conditions for protease production by Bacillus subtilis HB04. This approach facilitated the study of interaction of a large number of variables spanned by factors and their settings with a small number of experimental runs leading to considerable economy in time and cost for the process optimization. The objective of this research was to determine the significant parameters in the production of protease by Bacillus subtilis HB04 in submerged fermentation. Five factors viz., carbon, nitrogen, metal ions, temperature and agitation, each at four levels were selected and an orthogonal array layout of L16 (45) performed. The experiment result indicated that dextrose (1 %), (NH4)2PO4 (0.5 %), temperature (30oC) and agitation (100 rpm) were the important factors for protease production by Bacillus subtilis HB04 in submerged fermentation at the optimum levels.	en
heal.publisher	Νερατζής, Ηλίας	el
heal.publisher	Σιανούδης, Ιωάννης	el
heal.journalName	e-Journal of Science & Technology	en
heal.journalName	e-Περιοδικό Επιστήμης & Τεχνολογίας	el
heal.journalType	peer-reviewed
heal.fullTextAvailability	true