Determination of the sensitivity range of biuret test for undergraduate biochemistry experiments

dc.contributor.author	Janairo, Gerardo	en
dc.contributor.author	Linley, Marianne S.	en
dc.contributor.author	Yap, Leonisa	en
dc.contributor.author	Llanos-Lazaro, Nancy	en
dc.contributor.author	Robles, Julita	en
dc.date.accessioned	2015-02-03T09:50:21Z
dc.date.available	2015-02-03T09:50:21Z
dc.date.issued	2015-02-03
dc.identifier.uri	http://hdl.handle.net/11400/5532
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://e-jst.teiath.gr/	en
dc.subject	Protein quantitation
dc.subject	Ποσοτικοποίηση πρωτεϊνών
dc.subject	Biuret test
dc.subject	UV-Vis absorption spectroscopy
dc.subject	UV-Vis φασματοσκοπία απορρόφησης
dc.subject	Διουριτικός έλεγχος
dc.title	Determination of the sensitivity range of biuret test for undergraduate biochemistry experiments	en
heal.type	journalArticle
heal.classification	Technology
heal.classification	Chemical technology
heal.classification	Τεχνολογία
heal.classification	Χημική τεχνολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	http://skos.um.es/unescothes/C00565
heal.classificationURI	N/A-Τεχνολογία
heal.classificationURI	N/A-Χημική τεχνολογία
heal.language	en
heal.access	free
heal.publicationDate	2011
heal.bibliographicCitation	Janairo, G., Linley, M.S., Yap, L., Llanos - Lazaro, N. and Robles, J. (2011). Determination of the sensitivity range of biuret test for undergraduate biochemistry experiments. "e-Journal of Science & Technology". [Online] 6(5): 81-87. Available from: http://e-jst.teiath.gr/	en
heal.abstract	Quantitation of the total protein content in a sample is a critical step in protein analysis. Molecular UV-Vis absorption spectroscopy is very efficient in quantitative analysis such as protein quantitation and has extensive applications in chemical and clinical laboratories worldwide. Among the various techniques for protein assay, biuret test is of particular interest in this study. This study sought to verify the sensitivity of biuret assay to protein samples with concentrations ranging from 0.0100 to 5.00 mg/mL. Albumin chicken egg was used as the protein sample. The assay was assessed to determine the range of concentration in which it will show linearity. The biuret test exhibited linearity in a wide range of concentrations (0.1000 ± 0.0004 to 5.00 ± 0.02 mg/mL). The starting protein concentration range established for the calibration curve of this assay is lower than the found literature value (1 mg/sample). The study provides a more workable range of biuret test.	en
heal.publisher	Νερατζής, Ηλίας	el
heal.publisher	Σιανούδης, Ιωάννης	el
heal.journalName	e-Journal of Science & Technology	en
heal.journalName	e-Περιοδικό Επιστήμης & Τεχνολογίας	el
heal.journalType	peer-reviewed
heal.fullTextAvailability	true