Clinical-oriented polyphasic identification, typing and susceptibility methodology for Candida rugosa  isolates to establish potential virulence markers and processing guidelines

dc.contributor.author	Κόλλια, Κ.	el
dc.contributor.author	Καμπούρης, Μανούσος Ε.	el
dc.contributor.author	Μπελάζη, Μαρία	el
dc.contributor.author	Κουσσίδου-Ερεμόνδη, Τ.	el
dc.contributor.author	Ανδρεάδης, Δ.	el
dc.date.accessioned	2015-02-03T11:10:09Z
dc.date.available	2015-02-03T11:10:09Z
dc.date.issued	2015-02-03
dc.identifier.uri	http://hdl.handle.net/11400/5539
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://e-jst.teiath.gr/	en
dc.subject	PCR-RFLP
dc.subject	Bloodstream fungal infections
dc.subject	Candida rugosa
dc.subject	Μυκητιασικές λοιμώξεις κυκλοφορίας αίματος
dc.subject	TTC culture
dc.subject	TTC πολιτισμός
dc.subject	Electrophoretic karyotyping
dc.subject	Ηλεκτροφορητικό καρυότυπο
dc.title	Clinical-oriented polyphasic identification, typing and susceptibility methodology for Candida rugosa isolates to establish potential virulence markers and processing guidelines	en
dc.title	Πολυφασική μελετη ταυτοποίησης, τυποποίησης και προσδιορισμού ευαισθησίας στελεχών για προσδιορισμό δεικτών λοιμογονικότητας και ανάπτυξη εργαστηριακών διαδικασιών κλινικής εφαρμογής	el
heal.type	journalArticle
heal.classification	Science
heal.classification	Microbiology
heal.classification	Επιστήμες
heal.classification	Μικροβιολογία
heal.classificationURI	http://zbw.eu/stw/descriptor/15685-2
heal.classificationURI	http://skos.um.es/unescothes/C02502
heal.classificationURI	N/A-Επιστήμες
heal.classificationURI	N/A-Μικροβιολογία
heal.contributorName	Βελεγράκη, Αριστέα	el
heal.language	en
heal.access	free
heal.publicationDate	2012
heal.bibliographicCitation	Kollia, K., Kambouris, M.E., Belazi, M., Koussidou-Eremondi, T., Andreadis, D., et al. (2012). Clinical-oriented polyphasic identification, typing and susceptibility methodology for Candida rugosa isolates to establish potential virulence markers and processing guidelines. "e-Journal of Science & Technology". [Online] 7(1): 43-54. Available from: http://e-jst.teiath.gr/	en
heal.abstract	Candida rugosa is a rare as yet, though emerging pathogen, attracting increasing international interest. A collection of 10 clinical strains from the southern Balkan peninsula was subjected to polyphasic detection, identification and typing techniques, such as cultures in differential media, microscopy, assimilation tests, immunefluorescence, in vitro drug susceptibility (microdilution CLSI methodology and E-test) to multiple agents and both simple and amplification-based molecular techniques, such as electrophoretic karyotyping, RFLP-PFGE, PCR fingerprinting with mini- and microsatellite DNA, ribotyping and multiple PCR-RFLPs. Similarities and differences to other members of the genus were noted to determine appropriate identification, typing and treatment techniques and regimens for use in epidemiological studies and clinical applications, as the resulting C. rugosa profiles were in different degrees distinct but generally not dissimilar to other Candida species. TTC assimilation and Dalmau cultures allow identification, but typing is better achieved with electrophoretic karyotyping and specific PCR-RFLP, though the respective results are similar but not matching, indicating discontinuous intraspecific microevolutionary events.	en
heal.publisher	Νερατζής, Ηλίας	el
heal.publisher	Σιανούδης, Ιωάννης	el
heal.journalName	e-Journal of Science & Technology	en
heal.journalName	e-Περιοδικό Επιστήμης & Τεχνολογίας	el
heal.journalType	peer-reviewed
heal.fullTextAvailability	true