Cultivation of mushroom (Pleurotus ostreatus) and the microorganisms associated with the substrate used

dc.contributor.author	Obire, Omokaro	en
dc.contributor.author	Ogechi, Amadi Adiele	en
dc.date.accessioned	2015-02-12T00:10:38Z
dc.date.available	2015-02-12T00:10:38Z
dc.date.issued	2015-02-12
dc.identifier.uri	http://hdl.handle.net/11400/6083
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://e-jst.teiath.gr/	en
dc.subject	Mushroom
dc.subject	Pleurotus ostreatus
dc.subject	sawdust
dc.subject	Substrate
dc.subject	Fungi
dc.subject	Bacteria
dc.subject	E. coli
dc.subject	Μανιτάρι
dc.subject	Πριονίδια
dc.subject	Υπόστρωμα
dc.subject	Μύκητες
dc.subject	Βακτήρια
dc.title	Cultivation of mushroom (Pleurotus ostreatus) and the microorganisms associated with the substrate used	en
heal.type	journalArticle
heal.classification	Geography
heal.classification	Environmental sciences
heal.classification	Technology
heal.classification	Γεωγραφία
heal.classification	Περιβαλλοντικές επιστήμες
heal.classification	Τεχνολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85053986
heal.classificationURI	N/A-Environmental sciences
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	N/A-Γεωγραφία
heal.classificationURI	N/A-Περιβαλλοντικές επιστήμες
heal.classificationURI	N/A-Τεχνολογία
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85103515
heal.keywordURI	http://lod.nal.usda.gov/62479
heal.language	en
heal.access	free
heal.publicationDate	2013
heal.bibliographicCitation	Obire, O. and Ogechi, A.A. (2013). Cultivation of mushroom (Pleurotus ostreatus) and the microorganisms associated with the substrate used. "e-Journal of Science & Technology". [Online] 8(4): 49-59. Available from: http://e-jst.teiath.gr/	en
heal.abstract	This study was conducted to investigate the microorganisms associated with substrate used in the cultivation of mushroom (Pleurotus ostreatus) in a mushroom farm. The substrate which is a mixture of sawdust, rice bran, lime, and water was composted for 30 days and pasteurized for use in the cultivation. Samples of pasteurized substrates were randomly collected for the analyses using standard microbiological techniques. Microorganisms isolated and characterized and their frequency of isolation from the substrate include species of fungal genera Aspergillus (40.9%), Fusarium (22.7%), Mucor (5.6%) Penicillium (17.0%), Rhizopus (11.6%) and Trichoderma (2.3%). The species of bacteria genera isolated and frequency of isolation was Bacillus (36.67%) Clostridium (16.67%), Enterobacter (18.33%), Escherichia (15%), and Pseudomonas (13.33%). Aspergillus and Bacillus species had the highest frequency of occurrence for fungi and bacteria respectively. Proper composting promotes the development of a number of saprophytic soil microorganisms that helps in the degradation of the substrate. The presence of cellulolytic fungi such as Aspergillus, Penicillium and Trichoderma are associated with the composting process and do accelerate composting for efficient recycling. Generally, the presence of fungi and bacteria in the pasteurized substrate is attributed to the ineffectiveness of the method adopted for pasteurization and the possession of spores that were heat tolerant by most of the isolates. The presence of E. coli which is an indicator of faecal contamination is attributed to the feacal contamination of the streams and river banks around the Sawmill where logs are retained before being sawn with the sawdust as a by-product. The presence of potential pathogens such as Bacillus, Clostridium, E. coli and Enterobacter can lead to bacterial disease of edible mushrooms and economic loss. The presence of these microorganisms also has serious implications for human health.	en
heal.publisher	Νερατζής, Ηλίας	el
heal.publisher	Σιανούδης, Ιωάννης	el
heal.journalName	e-Journal of Science & Technology	en
heal.journalName	e-Περιοδικό Επιστήμης & Τεχνολογίας	el
heal.journalType	peer-reviewed
heal.fullTextAvailability	true