Collagen models as a probe in the decay of works of art

dc.contributor.author	Ζευγίτη, Στέλλα	el
dc.contributor.author	Σακαρέλλος, Κωνσταντίνος	el
dc.contributor.author	Σακαρέλλου-Δαϊτσιώτη, Μαρία	el
dc.contributor.author	Ιωακείμογλου, Ελένη Ε.	el
dc.contributor.author	Πάνου-Πομόνη, Ευγενία	el
dc.date.accessioned	2015-03-17T08:43:48Z
dc.date.available	2015-03-17T08:43:48Z
dc.date.issued	2015-03-17
dc.identifier.uri	http://hdl.handle.net/11400/8070
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://onlinelibrary.wiley.com/doi/10.1002/psc.823/abstract	el
dc.subject	Circular dichroism
dc.subject	Collagen model peptides
dc.subject	Μοντέλο κολλαγόνο πεπτίδια
dc.subject	Εγκύκλιος διχρωϊσμός
dc.subject	Immunological assays
dc.subject	Ανοσολογικές δοκιμασίες
dc.subject	Artificial ageing of collagen
dc.subject	Τεχνητή γήρανση του κολλαγόνου
dc.subject	Decay of works of art
dc.subject	Αποσύνθεση των έργων τέχνης
dc.title	Collagen models as a probe in the decay of works of art	en
heal.type	journalArticle
heal.secondaryTitle	synthesis, conformation and immunological studies	el
heal.classification	Science
heal.classification	Archaeology
heal.classification	Επιστήμες
heal.classification	Αρχαιολογία
heal.classificationURI	http://skos.um.es/unescothes/C03532
heal.classificationURI	http://skos.um.es/unescothes/C00213
heal.classificationURI	N/A-Επιστήμες
heal.classificationURI	N/A-Αρχαιολογία
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85026067
heal.language	en
heal.access	free
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας.Σχολή Καλλιτεχνικών Σπουδών.Τμήμα Συντήρησης Αρχαιοτήτων και Έργων Τέχνης	el
heal.publicationDate	2006-10
heal.bibliographicCitation	Zevgiti, S., Sakarellos, C., Sakarellos-Daitsiotis, M., Ioakimoglou, E. and Panou-Pomonis, E. (2006). Collagen models as a probe in the decay of works of art: synthesis, conformation and immunological studies. Journal of Peptide Science J. Pept. Sci.13 (2) pp 121-127. Received 7 July 2006. Revised 4 October 2006. [Accepted 17 October 2006].	en
heal.abstract	Proteinaceous substances such as collagen, casein and albumin have been widely used as binding media in a variety of works of art. Damages of these ‘sensitive’ materials, mainly caused of the influence of the environment, are responsible for the overall decay of works of art, and their identification is essential to understand ancient technologies, determine the extent of deterioration and help in future restoration and preservation processes. The most commonly used techniques for the identification of proteinaceous binding media are staining techniques, chromatography, spectrometry and immunological methods, although for the latter no systematic studies have been carried out until now. Aiming at contributing to the development of a reliable and reproducible immunoassay for the evaluation of the collagen-based decay of works of art, sequential polypeptides (Pro-X-Gly)n where X represents amino acid residues Val, Lys, Glu and (Hyp-Val-Gly)n were prepared as models of collagen fragments derived from artificially and naturally aged animal collagens. Conformational studies of the polypeptides by CD revealed the occurrence of polyproline II-like structures comparable with those of collagen. Polypeptides and collagen I were administered to animals, and the induced antibodies were used for the immunochemical detection and differentiation of collagen and collagen fragments. The combined application of (i) anti-collagen antibodies, which strongly interact with native collagen, but poorly recognized by artificially aged collagen and (ii) anti-polypeptide antibodies, which do not associate with native collagen, but are strongly recognized by collagen fragments in naturally or artificially aged collagen, is a valuable tool in determining the extent of decay in works of art. Copyright © 2006 European Peptide Society and John Wiley & Sons, Ltd.	en
heal.journalName	Journal of Peptide Science	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false