Application of the genotoxicity assay comet as a test for evaluating occupational exposure to chemotherapeutic agents

dc.contributor.author	Κυριακοπούλου, Αικατερίνη	el
dc.contributor.author	Γεωργοπούλου, Ελένη	el
dc.contributor.author	Εμμανουήλ, Χριστίνα	el
dc.contributor.author	Βάγκα, Ελισάβετ	el
dc.contributor.author	Μαχέρα, Κυριακή	el
dc.date.accessioned	2015-04-18T13:51:46Z
dc.date.issued	2015-04-18
dc.identifier.uri	http://hdl.handle.net/11400/8509
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.scopus.com/	en
dc.source	http://pharmakonpress.gr/	en
dc.subject	Εργαζόμενοι
dc.subject	Εργασία
dc.subject	Employees
dc.subject	Employment
dc.title	Application of the genotoxicity assay comet as a test for evaluating occupational exposure to chemotherapeutic agents	en
heal.type	journalArticle
heal.classification	Nursing
heal.classification	Therapeutics
heal.classification	Νοσηλευτική
heal.classification	Ιατρική περίθαλψη
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85093362
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85134723
heal.classificationURI	N/A-Νοσηλευτική
heal.classificationURI	N/A-Ιατρική περίθαλψη
heal.contributorName	Κωνσταντινίδης, Θεόδωρος, Κ.	el
heal.contributorName	Χατζάκη, Αικατερίνη	el
heal.dateAvailable	10000-01-01
heal.language	en
heal.access	forever
heal.recordProvider	Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Δημόσιας Υγείας και Κοινοτικής Υγείας. Κατεύθυνση Δημόσιας Υγείας (Δημόσιας Υγιεινής)	el
heal.publicationDate	2010-05
heal.bibliographicCitation	Kyriakopoulou, E., Georgopoulou, E., Emmanouil, C., Vagka, E., Machera, K., et. al (2010) Application of the genotoxicity assay comet as a test for evaluating occupational exposure to chemotherapeutic agents. "Review of Clinical Pharmacology and Pharmacokinetics", 24 (2), p. 162-164. Available from: http://www.scopus.com/record/display.url?eid=2-s2.0-77954422911&origin=resultslist&sort=plf-f&src=s&st1=Vagka+E&sid=F90CD4A1F71A25F269096E34A53FBFE3.53bsOu7mi7A1NSY7fPJf1g%3a20&sot=b&sdt=b&sl=20&s=AUTHOR-NAME%28Vagka+E%29&relpos=3&relpos=3&searchTerm=AUTHOR-NAME%28Vagka+E%29 [Accessed: 18/05/2015]	en
heal.abstract	The expansion of anti-cancer chemotherapy has led to a significant concern about the health and safety work conditions of the personnel handling these drugs that have been characterized by the International Agency for Research on Cancer as hazardous to human. However, no reliable monitoring test of occupational exposure is currently clinically applied for the medical surveillance of these employees. Based on a descriptive self-evaluation survey, we have previously shown inadequate health and safety design and practices related to chemotherapeutic drug handling in Greek hospitals. In the present study, we use a quantitative and objective genotoxicity method, the comet assay, to evaluate occupational exposure to chemotherapeutics. DNA single strand breaks as revealed by comet in peripheral mononuclear blood cells were significantly higher in a group of employees involved in the preparation, handling or administration of cancer chemotherapeutic agents that in a control group. Comet data were correlated to demographic/epidemiological health data of the subjects as well as to the self-evaluation subjective occupational exposure data. Our results demonstrate clearly genotoxic effects related to occupational exposure to chemotherapeutics, indicating insufficient safety design and practices. They also suggest that SSBs could be an appropriate biomarker for evaluating genotoxicity in these employees. The comet assay in peripheral blood could provide an easy tool of the Health and Safety Department in hospitals for the exposure monitoring.	en
heal.journalName	Review of Clinical Pharmacology and Pharmacokinetics	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false