Development and validation of an ion-pair RP-HPLC method for the determination of oligopeptide-20 in cosmeceuticals

dc.contributor.author	Παπαγιάννη, Π.	el
dc.contributor.author	Βαρβαρέσου, Αθανασία	el
dc.contributor.author	Παπαγεωργίου, Σπυρίδων	el
dc.contributor.author	Παντέρη, Ειρήνη	el
dc.date.accessioned	2015-04-28T16:43:41Z
dc.date.available	2015-04-28T16:43:41Z
dc.date.issued	2015-04-28
dc.identifier.uri	http://hdl.handle.net/11400/9197
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com	en
dc.subject	Καλλυντικά
dc.subject	Πειραματικές μέθοδοι
dc.subject	Experimental methods
dc.title	Development and validation of an ion-pair RP-HPLC method for the determination of oligopeptide-20 in cosmeceuticals	en
heal.type	journalArticle
heal.classification	Aesthetics
heal.classification	Chemistry
heal.classification	Αισθητική
heal.classification	Χημεία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85001441
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85022986
heal.classificationURI	N/A-Αισθητική
heal.classificationURI	N/A-Χημεία
heal.identifier.secondary	10.1016/j.jpba.2011.07.001
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθηνών. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Αισθητικής και Κοσμητολογίας	el
heal.publicationDate	2011
heal.bibliographicCitation	Papagianni, P., Varvaresou, A., Papageorgiou, S. and Panderi, I. (2011) Development and validation of an ion-pair RP-HPLC method for the determination of oligopeptide-20 in cosmeceuticals. "Journal of Pharmaceutical and Biomedical Analysis", 56 (3), p.645-649	en
heal.abstract	Oligopeptide-20 is a growth-factor mimicking peptide used in cosmeceuticals. This article describes the development and validation of an ion-pair reversed-phase liquid chromatography method that allows, after liquid–liquid extraction, the quantification of oligopeptide-20 in cosmetic creams. Chromatographic separation was achieved on a cyanopropyl Hypersil analytical column (100 mm × 2.1 mm i.d., 5 μm particle size), using a mobile phase of acetonitrile–heptafluorobutyric acid (pH = 2.5, 9.0 mM) (70:30, v/v) containing 0.045% diethylamine at a flow rate of 0.50 mL min−1. Ultraviolet (UV) spectrophotometric detection at 225 nm was used. The method had linear calibration curve over the range 1.35–4.95 μg mL−1 for oligopeptide-20. The intra- and inter-day RSD values were less than 3.3%, while the relative percentage error, %Er, was less than 1.9. The developed method was applied successfully to the quality control of a cosmetic cream containing 0.003% (w/w) oligopeptide-20.	en
heal.journalName	Journal of Pharmaceutical and Biomedical Analysis	el
heal.journalType	peer-reviewed
heal.fullTextAvailability	false