Development and validation of a reversed-phase ion-pair liquid chromatography method for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams

dc.contributor.author	Βαρβαρέσου, Αθανασία	el
dc.contributor.author	Τσιριβάς, Ευθύμιος	el
dc.contributor.author	Ιακώβου, Κρίτων	el
dc.contributor.author	Γκίκας, Ευάγγελος	el
dc.contributor.author	Παπαθωμάς, Ζαχαρίας	el
dc.date.accessioned	2015-04-29T14:25:55Z
dc.date.available	2015-04-29T14:25:55Z
dc.date.issued	2015-04-29
dc.identifier.uri	http://hdl.handle.net/11400/9256
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com	en
dc.source	http://www.sciencedirect.com/science/article/pii/S0003267006001929	en
dc.subject	Καλλυντικά
dc.subject	Χημικά παρασκευάσματα
dc.subject	Φαρμακευτική
dc.subject	Chemical preparations
dc.subject	Pharmacy
dc.title	Development and validation of a reversed-phase ion-pair liquid chromatography method for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams	en
heal.type	journalArticle
heal.classification	Aesthetics
heal.classification	Cosmetics
heal.classification	Αισθητική
heal.classification	Κοσμητολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85001441
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85033139
heal.classificationURI	N/A-Αισθητική
heal.classificationURI	N/A-Κοσμητολογία
heal.contributorName	Βοναπάρτη, Αριάδνη	el
heal.contributorName	Παντέρη, Ειρήνη	el
heal.identifier.secondary	10.1016/j.aca.2005.12.074
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθηνών. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Αισθητικής και Κοσμητολογίας	el
heal.publicationDate	2006-02-23
heal.bibliographicCitation	Varvaresou, A., Tsirivas, E., Iakovou, K., Gikas, E., Papathomas, Z. et al. (2006) Development and validation of a reversed-phase ion-pair liquid chromatography method for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams. "Analytica Chimica Acta" 573-574, p.284-290	en
heal.abstract	A reversed-phase ion-pair high-performance liquid chromatographic method was developed and validated for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creams. The determination was performed on a BDS C 18 analytical column (250 × 4.6 mm i.d., 5 μm particle size); the mobile phase consisted of 0.020 M tetrabutylammonium hydroxide and 0.025 M potassium dihydrogen phosphate (pH 6.8) mixed with acetonitrile in a ratio (77:23, v/v) and pumped at a flow rate 1.00 ml min−1. The UV detector was operated at 260 nm. The retention times of the magnesium ascorbyl phosphate, melatonin and chlorthalidone that was used as internal standard, were 6.55, 9.18 and 11.07 min, respectively. Calibration graphs are linear (r better than 0.9990, n = 6), in concentration range 1.00–10.00 μg ml−1 for magnesium ascorbyl phosphate and 0.63–6.25 μg ml−1 for melatonin. The intra- and inter-day R.S.D. values were less than 6.0%, while the relative percentage error Er was less than 3.5% (n = 5). The quantitation limits were 0.69 and 0.47 μg ml−1, for magnesium ascorbyl phosphate and melatonin, respectively. The method was applied to the analysis of a cosmetic cream and proved to be suitable for rapid and reliable quality control.	en
heal.publisher	Elsevier	en
heal.journalName	Analytica Chimica Acta	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true