Equivalency testing of TTC Tergitol 7 agar (ISO 9308-1:2000) with five culture media for the detection of E. coli in water samples in Greece

dc.contributor.author	Μαυρίδου, Αθηνά	el
dc.contributor.author	Σμέτη, Ελένη	el
dc.contributor.author	Μανδηλαρά, Γεωργία	el
dc.contributor.author	Μπούφα, Π.	el
dc.contributor.author	Αρβανιτίδου-Βαγιωνά, Μαλαματένια	el
dc.date.accessioned	2015-05-01T20:05:41Z
dc.date.available	2015-05-01T20:05:41Z
dc.date.issued	2015-05-01
dc.identifier.uri	http://hdl.handle.net/11400/9435
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.iwaponline.com/wst/06101/wst061010067.htm	en
dc.subject	δοκιμές ισοδυναμίας
dc.subject	Εσερίχια κόλι
dc.subject	μικροβιολογικές μεθόδους
dc.subject	νερό
dc.subject	Ελλάδα
dc.subject	equivalency testing
dc.subject	Escherichia coli
dc.subject	microbiological methods
dc.subject	water
dc.subject	Greece
dc.title	Equivalency testing of TTC Tergitol 7 agar (ISO 9308-1:2000) with five culture media for the detection of E. coli in water samples in Greece	en
heal.type	journalArticle
heal.classification	Τεχνολογία
heal.classification	Μικροβιολογία
heal.classification	Technology
heal.classification	Microbiology
heal.classificationURI	N/A-Τεχνολογία
heal.classificationURI	N/A-Μικροβιολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85133147
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh00005932
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85044799
heal.keywordURI	http://zbw.eu/stw/descriptor/17184-0
heal.contributorName	Βανταράκης, Απόστολος	el
heal.contributorName	Βασιλαντωνοπούλου, Γ.	el
heal.contributorName	Καραούλη, Βασιλική	el
heal.identifier.secondary	DOI: 0.2166/wst.2010.781
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2010
heal.bibliographicCitation	Mavridou, A., Smeti, E., Mandilara, G., Boufa, P., Vagiona-Arvanitidou, M. et al. (2010) Equivalency testing of TTC Tergitol 7 agar (ISO 9308-1:2000) with five culture media for the detection of E. coli in water samples in Greece. Water Science and Technology. [Online] 61 (1), pp.67-76. Available from: http://www.iwaponline.com [Accessed 01/05/2015]	en
heal.abstract	In this study ten laboratories in Greece compared the performance of reference method TTC Tergitol 7 Agar (with the additional test of b-glucuronidase production) with five alternative methods, to detect E. coli in water, in line with European Water Directive recommendations. The samples were prepared by spiking drinking water with sewage effluent following a standard protocol. Chlorinated and non-chlorinated samples were used. The statistical analysis was based on the mean relative difference of confirmed counts and was performed in line with ISO 17994. The results showed that in total, three of the alternative methods (Chromocult Coliform agar, Membrane Lauryl Sulfate agar and Trypton Bilex-glucuronidase medium) were not different from TTC Tergitol 7 agar (TTC Tergitol 7 agar vs Chromocult Coliform agar, 294 samples, mean RD% 5.55; vs MLSA, 302 samples, mean RD% 1; vs TBX, 297 samples, mean RD% -2.78). The other two alternative methods (Membrane Faecal coliform medium and Colilert 18/ Quantitray) gave significantly higher counts than TTC Tergitol 7 agar (TTC Tergitol 7 agar vs MFc, 303 samples, mean RD% 8.81; vs Colilert-18/Quantitray, 76 samples, mean RD% 18.91). In other words, the alternative methods generated performance that was as reliable as, or even better than, the reference method. This study will help laboratories in Greece overcome culture and counting problems deriving from the EU reference method for E. coli counts in water samples.	en
heal.publisher	IWA Publishing	en
heal.journalName	Water Science and Technology	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true