Membrane protein carbonylation in non-leucodepleted CPDA-preserved red blood cells

dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Οικονόμου-Petersen, Ευφροσύνη	el
dc.contributor.author	Μαργαρίτης, Λουκάς Χ.	el
dc.date.accessioned	2015-05-06T18:49:55Z
dc.date.available	2015-05-06T18:49:55Z
dc.date.issued	2015-05-06
dc.identifier.uri	http://hdl.handle.net/11400/9827
dc.rights	An error occurred on the license name.	*
dc.rights.uri	An error occurred getting the license - uri.	*
dc.source	http://www.sciencedirect.com/science/article/pii/S1079979606000337	en
dc.subject	μεμβρανικές πρωτεΐνες
dc.subject	αποθήκευση
dc.subject	καρβονυλίωση
dc.subject	οξείδωση
dc.subject	Membrane proteins
dc.subject	Storage
dc.subject	Carbonylation
dc.subject	Oxidation, Physiological
dc.title	Membrane protein carbonylation in non-leucodepleted CPDA-preserved red blood cells	en
heal.type	journalArticle
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85083471
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh00008427
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85096309
heal.contributorName	Παπασιδέρη, Ισιδώρα Σ.	el
heal.identifier.secondary	DOI: 10.1016/j.bcmd.2006.01.003
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2006-03
heal.bibliographicCitation	Kriebardis, Α., Antonelou, Μ.Η., Stamoulis, Κ.Ε., Economou-Petersen, Ε., Margaritis, L.H, et al. (2006) Membrane protein carbonylation in non-leukodepleted CPDA-preserved red blood cells. Blood Cells Molecules and Diseases. [Online] 36 (2), pp.279-282. Available from:http://www.sciencedirect.com [Accessed 06/05/2015]	en
heal.abstract	Transfusion of allogeneic blood products is associated with adverse reactions and complications. Some of the negative effects of RBC transfusion are associated with the storage lesion. The importance of RBC oxidative damage in the storage lesion is not well documented. We monitored the storage-induced membrane protein oxidation in CPDA-preserved non-leukodepleted RBCs units from five blood donors in the course of the storage period, as assessed by protein carbonylation levels estimation. Carbonylated protein content was determined following 2,4-dinitrophenylhydrazine derivatization and SDS-polyacrylamide gel electrophoresis coupled with Western blotting. Immunoblotting with dinitrophenol-specific antibody revealed increased RBC membrane protein carbonyls with prolonged storage in CPDA units. This finding supports the idea of oxidation as a part of the storage lesion.	en
heal.publisher	Elsevier	en
heal.journalName	Blood Cells Molecules and Diseases	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false