Progressive oxidation of cytoskeletal proteins and accumulation of denatured hemoglobin in stored red cells

dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Οικονόμου-Petersen, Ευφροσύνη	el
dc.contributor.author	Μαργαρίτης, Λουκάς Χ.	el
dc.date.accessioned	2015-05-06T19:35:49Z
dc.date.available	2015-05-06T19:35:49Z
dc.date.issued	2015-05-06
dc.identifier.uri	http://hdl.handle.net/11400/9829
dc.rights	An error occurred on the license name.	*
dc.rights.uri	An error occurred getting the license - uri.	*
dc.source	http://www.ncbi.nlm.nih.gov/pubmed/17367509	en
dc.subject	ερυθρά αιμοσφαίρια
dc.subject	κυτταροσκελετός
dc.subject	αποθήκευση
dc.subject	δεσμευμένη αιμοσφαιρίνη σε μεμβράνη
dc.subject	οξείδωση
dc.subject	καρβονυλίωση
dc.subject	red blood cell
dc.subject	Cytoskeleton
dc.subject	Storage
dc.subject	membrane-bound hemoglobin
dc.subject	Oxidation, Physiological
dc.subject	carbonylation
dc.title	Progressive oxidation of cytoskeletal proteins and accumulation of denatured hemoglobin in stored red cells	en
heal.type	journalArticle
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85035256
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh00008427
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85096309
heal.contributorName	Παπασιδέρη, Ισιδώρα Σ.	el
heal.identifier.secondary	DOI: 10.1111/j.1582-4934.2007.00008.x
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2007-01
heal.bibliographicCitation	Kriebardis, Α., Antonelou, Μ.Η., Stamoulis, Κ.Ε., Economou-Petersen, Ε., Margaritis, L.H, et al. (2007) Progressive oxidation of cytoskeletal proteins and accumulation of denatured hemoglobin in stored red cells. Journal of Cellular and Molecular Medicine. [Online] 11 (1), pp.148-155. Available from: http://www.ncbi.nlm.nih.gov [Accessed 06/05/2015]	en
heal.abstract	Red blood cell (RBC) membrane proteins undergo progressive pathological alterations during storage. In conditions of increased cellular stress, the cytoskeleton also sustains certain modifications. The hemoglobin (Hb) content and oxidative status of the RBC cytoskeletons as a function of the storage period remain unclear. The possible Hb content and oxidative alterations occurring in the cytoskeletons in the course of storage were monitored in six units, by means of electrophoresis, immunoblotting and protein carbonylation assays. A proportion of the ghost-bound Hb consists of non-reducible crosslinkings of probably oxidized(denatured Hb or hemichromes.The defective Hb-membrane association was strongly affected by the prolonged storage. A progressive accumulation of Hb monomers, multimers and high molecular weight aggregates to corresponding cytoskeletons were also evident. The oxidative index of the cytoskeletal proteins was found increased, signalizing oxidative modifications in spectrin and possibly other cytoskeletal proteins. The reported data corroborate the evidence for oxidative damage in membrane proteins with emphasis to the cytoskeletal components. They partially address the pathophysiological mechanisms underlying the RBC storage lesion, add some new insight in the field of RBC storage as a hemoglobin- and cytoskeleton-associated pathology and suggest the possible use of antioxidants in the units intended for transfusion.	en
heal.journalName	Journal of Cellular and Molecular Medicine	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false