Storage-dependent remodeling of the red blood cell membrane is associated with increased immunoglobulin G binding, lipid raft rearrangement, and caspase activation

dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Οικονόμου-Petersen, Ευφροσύνη	el
dc.contributor.author	Μαργαρίτης, Λουκάς Χ.	el
dc.date.accessioned	2015-05-06T19:50:42Z
dc.date.available	2015-05-06T19:50:42Z
dc.date.issued	2015-05-06
dc.identifier.uri	http://hdl.handle.net/11400/9831
dc.rights	An error occurred on the license name.	*
dc.rights.uri	An error occurred getting the license - uri.	*
dc.source	http://onlinelibrary.wiley.com/doi/10.1111/j.1537-2995.2007.01254.x/full	en
dc.subject	αποθήκευση
dc.subject	ερυθρά αιμοσφαίρια
dc.subject	ανοσοσφαιρίνη
dc.subject	ενεργοποίηση της κασπάσης
dc.subject	αναδιάταξη
dc.subject	Storage
dc.subject	red blood cell
dc.subject	Immunoglobulin G
dc.subject	caspase activation
dc.subject	Rearrangements (Chemistry)
dc.title	Storage-dependent remodeling of the red blood cell membrane is associated with increased immunoglobulin G binding, lipid raft rearrangement, and caspase activation	en
heal.type	journalArticle
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh00008427
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85064565
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85111787
heal.contributorName	Παπασιδέρη, Ισιδώρα Σ.	el
heal.identifier.secondary	DOI: 10.1111/j.1537-2995.2007.01254.x
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2007-07
heal.bibliographicCitation	Kriebardis, Α., Antonelou, Μ.Η., Stamoulis, Κ.Ε., Economou-Petersen, Ε., Margaritis, L.H, et al. (2007) Storage-dependent remodeling of the red blood cell membrane is associated with increased immunoglobulin G binding, lipid raft rearrangement, and caspase activation. Transfusion. [Online] 47 (7), pp.1112-1120. Available from: http://onlinelibrary.wiley.com [Accessed 06/05/2015]	en
heal.abstract	BACKGROUND: The elucidation of the storage lesion is important for the improvement of red blood cell (RBC) storage. Ex vivo storage is also a model system for studying cell-signaling events in the senescence and programmed cell death of RBCs. The membrane hosts critical steps in these mechanisms and undergoes widespread remodeling over the storage period. STUDY DESIGN AND METHODS: Fresh and CPDA-stored RBCs from 21 blood donors were evaluated as whole cells, membrane ghosts, and cytoskeletons by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, immunoblotting, immunofluorescence microscopy, and in situ assays. Band 3 content, immunoglobulin G (IgG) content, specific protein movement to and from the membrane, and caspase system activation were measured. RESULTS: During storage, Band 3 protein was aggregated and its content decreased as did the content of several lipid raft–related proteins. IgG binding to the membrane increased. Sorcin and synexin moved from the cytosol to the membrane, stomatin and flotillins left the membrane, the Fas protein was oligomerized, and caspase was activated. CONCLUSION: The remodeling of the RBC membrane during storage includes loss and oxidative cross-linking of Band 3 as well as IgG binding. This process occurs with lipid raft development and loss and is probably driven by caspase activation. Oxidative injury appears to be an important driver of RBC aging during storage.	en
heal.journalName	Transfusion	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false