RBC-derived vesicles during storage

dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Οικονόμου-Petersen, Ευφροσύνη	el
dc.contributor.author	Μαργαρίτης, Λουκάς Χ.	el
dc.date.accessioned	2015-05-06T20:05:16Z
dc.date.available	2015-05-06T20:05:16Z
dc.date.issued	2015-05-06
dc.identifier.uri	http://hdl.handle.net/11400/9833
dc.rights	An error occurred on the license name.	*
dc.rights.uri	An error occurred getting the license - uri.	*
dc.source	http://onlinelibrary.wiley.com/doi/10.1111/j.1537-2995.2008.01794.x/pdf	en
dc.subject	κυστίδια
dc.subject	αποθήκευση
dc.subject	υπερδομή
dc.subject	σύνθεση πρωτεΐνης
dc.subject	οξείδωση
dc.subject	συστατικά σηματοδότησης
dc.subject	vesicles
dc.subject	Storage
dc.subject	Ultrastructure (Biology)
dc.subject	protein composition
dc.subject	Oxidation, Physiological
dc.subject	signaling components
dc.title	RBC-derived vesicles during storage	en
heal.type	journalArticle
heal.secondaryTitle	ultrastructure, protein composition, oxidation, and signaling components	en
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh00008427
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85139504
heal.keywordURI	http://lod.nal.usda.gov/7747
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85096309
heal.contributorName	Παπασιδέρη, Ισιδώρα Σ.	el
heal.identifier.secondary	DOI: 10.1111/j.1537-2995.2008.01794.x
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2008-06
heal.bibliographicCitation	Kriebardis, Α., Antonelou, Μ.Η., Stamoulis, Κ.Ε., Economou-Petersen, Ε., Margaritis, L.H, et al. (2008) RBC-derived vesicles during storage: ultrastructure, protein composition, oxidation, and signaling components. Transfusion. [Online] 48 (9), pp.1943-1953. Available from: http://onlinelibrary.wiley.com [Accessed 06/05/2015]	en
heal.abstract	BACKGROUND: Red cells (RBCs) lose membrane in vivo, under certain conditions in vitro, and during the ex vivo storage of whole blood, by releasing vesicles. The vesiculation of the RBCs is a part of the storage lesion. The protein composition of the vesicles generated during storage of banked RBCs has not been studied in detail. STUDY DESIGN AND METHODS: Vesicles were isolated from the plasma of nonleukoreduced RBC units in citrate-phosphate-dextrose-adenine, at eight time points of the storage period and shortly afterward. The degree of vesiculation, ultrastructure, oxidation status, and protein composition of the vesicles were evaluated by means of electron microscopy and immunoblotting. RBCs and ghost membranes were investigated as controls. RESULTS: The total protein content of the vesicle fraction and the size of the vesicles increased but their structural integrity decreased over time. The oxidation index of the vesicles released up to Day 21 of storage was greater than that of the membrane ghosts of the corresponding intact RBCs. The vesicles contain aggregated hemoglobin, band 3, and lipid raft proteins, including flotillins. They also contain Fas, FADD, procaspases 3 and 8, caspase 8 and caspase 3 cleavage products (after the 10th day), CD47 (after the 17th day), and immunoglobulin G. CONCLUSION: These data indicate that the vesicles released during storage of RBCs contain lipid raft proteins and oxidized or reactive signaling components commonly associated with the senescent RBCs. Vesiculation during storage of RBCs may enable the RBC to shed altered or harmful material.	en
heal.journalName	Transfusion	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false