Red blood cell aging markers during storage in
citrate-phosphate-dextrose–saline-adenine-glucose-mannitol

dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Οικονόμου-Petersen, Ευφροσύνη	el
dc.contributor.author	Μαργαρίτης, Λουκάς Χ.	el
dc.date.accessioned	2015-05-06T20:24:51Z
dc.date.available	2015-05-06T20:24:51Z
dc.date.issued	2015-05-06
dc.identifier.uri	http://hdl.handle.net/11400/9834
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://onlinelibrary.wiley.com/doi/10.1111/j.1537-2995.2009.02449.x/pdf	en
dc.subject	ερυθρά αιμοσφαίρια
dc.subject	αποθήκευση
dc.subject	κιτρικό άλας
dc.subject	φωσφορικό άλας
dc.subject	αμυλοσάκχαρο
dc.subject	αλατόνερο
dc.subject	αδενίνη
dc.subject	γλυκόζη
dc.subject	μαννιτόλη
dc.subject	Red blood cell
dc.subject	Storage
dc.subject	citrate (si)-synthase
dc.subject	phosphate
dc.subject	Dextrose
dc.subject	saline
dc.subject	adenine
dc.subject	glucose
dc.subject	Mannitol
dc.title	Red blood cell aging markers during storage in citrate-phosphate-dextrose–saline-adenine-glucose-mannitol	en
heal.type	journalArticle
heal.classification	Χημεία
heal.classification	Κυτταρολογία
heal.classification	Chemistry
heal.classification	Cell biology
heal.classificationURI	N/A-Χημεία
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://skos.um.es/unescothes/C00568
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh00008427
heal.keywordURI	http://lod.nal.usda.gov/4005
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85037427
heal.keywordURI	http://lod.nal.usda.gov/4103
heal.keywordURI	http://lod.nal.usda.gov/6220
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh2008008250
heal.contributorName	Παπασιδέρη, Ισιδώρα Σ.	el
heal.identifier.secondary	DOI: 10.1111/j.1537-2995.2009.02449.x
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2010-02
heal.bibliographicCitation	Kriebardis, Α., Antonelou, Μ.Η., Stamoulis, Κ.Ε., Economou-Petersen, Ε., Margaritis, L.H, et al. (2009) Red blood cell aging markers during storage in citrate-phosphate-dextrose–saline-adenine-glucose-mannitol. Transfusion. [Online] 50 (2), pp.376-389. Available from: http://onlinelibrary.wiley.com [Accessed 06/05/2015]	en
heal.abstract	BACKGROUND: It has been suggested that red blood cell (RBC) senescence is accelerated under blood bank conditions, although neither protein profile of RBC aging nor the impact of additive solutions on it have been studied in detail. STUDY DESIGN AND METHODS: RBCs and vesicles derived from RBCs in both citrate-phosphate-dextrose (CPD)–saline-adenine-glucose-mannitol (SAGM) and citrate-phosphate-dextrose-adenine (CPDA) were evaluated for the expression of cell senescence markers (vesiculation, protein aggregation, degradation, activation, oxidation, and topology) through immunoblotting technique and immunofluorescence or immunoelectron microscopy study. RESULTS: A group of cellular stress proteins exhibited storage time– and storage medium–related changes in their membrane association and exocytosis. The extent, the rate, and the expression of protein oxidation, Fas oligomerization, caspase activation, and protein modifi- cations in Band 3, hemoglobin, and immunoglobulin G were less conspicuous and/or exhibited significant time retardation under storage in CPD-SAGM, compared to the CPDA storage. There was evidence for the localization of activated caspases near to the membrane of both cells and vesicles. CONCLUSIONS: We provide circumstantial evidence for a lower protein oxidative damage in CPD-SAGM– stored RBCs compared to the CPDA-stored cells. The different expression patterns of the senescence markers in the RBCs seem to be accordingly related to the oxidative stress management of the cells. We suggest that the storage of RBCs in CPD-SAGM might be more alike the in vivo RBC aging process, compared to storage in CPDA, since it is characterized by a slower stimulation of the recognition signaling pathways that are already known to trigger the erythrophagocytosis of senescent RBCs.	en
heal.journalName	Transfusion	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false