Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis

dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Βελέντζας, Αθανάσιος	el
dc.contributor.author	Κόκκαλης, Απόστολος	el
dc.contributor.author	Γεωργακοπούλου, Σοφία-Χριστίνα	el
dc.date.accessioned	2015-05-06T21:20:16Z
dc.date.available	2015-05-06T21:20:16Z
dc.date.issued	2015-05-07
dc.identifier.uri	http://hdl.handle.net/11400/9836
dc.rights	An error occurred on the license name.	*
dc.rights.uri	An error occurred getting the license - uri.	*
dc.source	http://www.sciencedirect.com/science/article/pii/S1874391911001515	en
dc.subject	Νεφρική νόσο τελικού σταδίου
dc.subject	αιμοκάθαρση
dc.subject	ερυθρά αιμοσφαίρια
dc.subject	Μεμβράνη πρωτέωμα
dc.subject	παραγωγή
dc.subject	End stage renal disease
dc.subject	Hemodialysis
dc.subject	Red blood cell
dc.subject	Membrane proteome
dc.subject	ROS production
dc.title	Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis	en
heal.type	journalArticle
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85060189
heal.contributorName	Παπασιδέρη, Ισιδώρα Σ.	el
heal.identifier.secondary	DOI: 10.1016/j.jprot.2011.04.009
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2011-10-19
heal.bibliographicCitation	Antonelou, Μ.Η., Kriebardis, Α., Velentzas, A.D., Kokkalis, A.C, Georgakopoulou, S.C. et al. (2011) Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis. Journal of Proteomics. [Online] 74 (11), pp.2441-2452. Available from: http://www.sciencedirect.com [Accessed 06/05/2015]	en
heal.abstract	This study was designed to evaluate the oxidative stress status of erythrocytes and its association with cellular ultrastructure and membrane proteome modifications in patients with end stage renal disease (ESRD) on hemodialysis (HD). For that purpose, we studied red blood cells' (RBCs) modifications in twelve non-diabetic ESRD patients that were responsive in erythropoietin therapy. Intracellular ROS levels were measured by fluorometry, RBCs ultra-structure was examined by electron microscopy, while the membrane proteome by electrophoresis and immunoblotting. Compared to the healthy subjects, the uremic RBCs exhibited significantly increased ROS accumulation. Dialysis partially ameliorated the basal ROS levels but triggered cellular sensitivity to exogenous oxidative stimuli. Common membrane modifications involved loss, aggregation, fragmentation and carbonylation of critical components as well as over-expression of stress markers. HD significantly contributed to membrane proteome remodeling, especially for aquaporin-1, peroxiredoxin-2 and ubiquitinated proteins. The intracellular redox status and the closely associated membrane modifications seemed to be related to membrane instability, loss of surface area through vesiculation, echinocytosis and stomatocytosis. Our data evinced a network of interactions among the uremic toxins, the RBCs membrane composition and the cellular shape modifications in ESRD, which is developed around a core of oxidative provocations and cellular responses.	en
heal.publisher	Elsevier	en
heal.journalName	Journal of Proteomics	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false