High level of heterogeneity among listeria monocytogenes isolates from clinical and food origin specimens in Greece

dc.contributor.author	Χούχουλα, Δήμητρα, Π.	el
dc.contributor.author	Πειρασμάκη, Δήμητρα	el
dc.contributor.author	Κοντελές, Σπυρίδων Ι.	el
dc.contributor.author	Κίζης, Δημοσθένης	el
dc.contributor.author	Κουσίσης, Σταμάτης	el
dc.date.accessioned	2015-05-07T16:54:19Z
dc.date.issued	2015-05-07
dc.identifier.uri	http://hdl.handle.net/11400/9840
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.liebertpub.com/fpd	en
dc.subject	Lab research
dc.subject	Greece
dc.subject	Food bacteria
dc.subject	Εργαστηριακή έρευνα
dc.subject	Ελλάδα
dc.subject	Βακτήρια φαγητού
dc.title	High level of heterogeneity among listeria monocytogenes isolates from clinical and food origin specimens in Greece	en
heal.type	journalArticle
heal.classification	Medicine
heal.classification	Microbiology
heal.classification	Ιατρική
heal.classification	Μικροβιολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh00006614
heal.classificationURI	http://skos.um.es/unescothes/C02502
heal.classificationURI	N/A-Ιατρική
heal.classificationURI	N/A-Μικροβιολογία
heal.keywordURI	http://zbw.eu/stw/descriptor/17184-0
heal.contributorName	Μπρατάκος, Μιχάλης, Σ.	el
heal.contributorName	Πόγγας, Νικόλαος	el
heal.contributorName	Χάρβαλου, Αικατερίνη	el
heal.contributorName	Τσακρής, Αθανάσιος	el
heal.identifier.secondary	DOI: 10.1089/fpd.2012.1187
heal.dateAvailable	10000-01-01
heal.language	en
heal.access	forever
heal.recordProvider	Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2012-09-06
heal.bibliographicCitation	Houhoula, D., Peirasmaki, D., Konteles, S., Kizis, D., Koussissis, S., et al. (2012) High level of heterogeneity among listeria monocytogenes isolates from clinical and food origin specimens in Greece. "Foodborne Pathogens and Disease", 9 (9), p. 848-852. Available from: http://online.liebertpub.com/doi/abs/10.1089/fpd.2012.1187 [Accessed: 07/05/2015].	en
heal.abstract	In order to examine the genetic variation of clinical and food isolates of Listeria monocytogenes in Greece, a total of 61 L. monocytogenes non-duplicate isolates, recovered from clinical specimens (n=19) and food (n=42), were serotyped and genotyped using two different Random Amplification of Polymorphic DNA (RAPD) protocols and Multiple Locus Variable Number Tandem Repeat Analysis (MLVA). Serotype group 4b, 4d, 4e prevailed (39.4%), among both clinical and food isolates, followed by serotype group 1/2a, 3a (23.0%), which nevertheless was detected only among food isolates. The most discriminatory typing protocol was MLVA, which grouped four isolates into two pairs, while the remaining isolates produced unique fingerprints. Similar results were obtained when taking into account the combination of the two RAPD protocols (Simpson index 0.999); six isolates were grouped into three pairs, two of which were the pairs that were identified also by MLVA. Single use of each RAPD protocol resulted in inferior discrimination (Simpson index 0.978 and 0.997, respectively). In conclusion, the two molecular procedures, MLVA, and the combined RAPD protocols, produced similar results, showing that L. monocytogenes isolates from clinical and food specimens were highly heterogenous and that clustering was very uncommon.	en
heal.publisher	Oliver, Stephen P.	en
heal.journalName	Foodborne Pathogens and Disease	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false