Apolipoprotein J/Clusterin is a novel structural component of human erythrocytes and a biomarker of cellular stress and senescence

dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Τρουγκάκος, Ιωάννης Π.	el
dc.contributor.author	Παπασιδέρη, Ισιδώρα Σ.	el
dc.date.accessioned	2015-05-07T19:21:01Z
dc.date.available	2015-05-07T19:21:01Z
dc.date.issued	2015-05-07
dc.identifier.uri	http://hdl.handle.net/11400/9895
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0026032	en
dc.subject	απολιποπρωτεΐνη
dc.subject	δομικό συστατικό
dc.subject	ερυθροκύτταρα
dc.subject	βιοδείκτης
dc.subject	Κυτταρικό στρες
dc.subject	κυτταρική τοπολογία
dc.subject	γήρας
dc.subject	apolipoprotein C
dc.subject	Structural Component
dc.subject	Erythrocytes
dc.subject	biomarkers
dc.subject	Cellular Stress
dc.subject	ellular topology
dc.subject	Senescence
dc.title	Apolipoprotein J/Clusterin is a novel structural component of human erythrocytes and a biomarker of cellular stress and senescence	en
heal.type	journalArticle
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://lod.nal.usda.gov/136430
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85044747
heal.keywordURI	http://lod.nal.usda.gov/17262
heal.keywordURI	http://skos.um.es/unesco6/610803
heal.identifier.secondary	DOI: 10.1371/journal.pone.0026032
heal.language	en
heal.access	free
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2011-10-06
heal.bibliographicCitation	Antonelou, M.H., Kriebardis, A.G., Stamoulis, K.E., Trougakos, I.P. and Papassideri, I.S. (2011) Apolipoprotein J/Clusterin Is a Novel Structural Component of Human Erythrocytes and a Biomarker of Cellular Stress and Senescence. PLoS ONE. [Online] 6 (10), e26032. Available from: http://journals.plos.org [Accessed 07/05/2015]	en
heal.abstract	Background: Secretory Apolipoprotein J/Clusterin (sCLU) is a ubiquitously expressed chaperone that has been functionally implicated in several pathological conditions of increased oxidative injury, including aging. Nevertheless, the biological role of sCLU in red blood cells (RBCs) remained largely unknown. In the current study we identified sCLU as a component of human RBCs and we undertook a detailed analysis of its cellular topology. Moreover, we studied the erythrocytic membrane sCLU content during organismal aging, in conditions of increased organismal stress and accelerated RBCs senescence, as well as during physiological in vivo cellular senescence. Methodology/Principal Findings: By using a combination of molecular, biochemical and high resolution microscopical methods we found that sCLU is a novel structural component of RBCs extra- and intracellular plasma membrane and cytosol. We observed that the RBCs membrane-associated sCLU decreases during organismal aging or exposure to acute stress (e.g. smoking), in patients with congenital hemolytic anemia, as well as during RBCs in vivo senescence. In all cases, sCLU reduction paralleled the expression of typical cellular senescence, redox imbalance and erythrophagocytosis markers which are also indicative of the senescence- and oxidative stress-mediated RBCs membrane vesiculation. Conclusions/Significance: We propose that sCLU at the mature RBCs is not a silent remnant of the erythroid precursors, but an active component being functionally implicated in the signalling mechanisms of cellular senescence and oxidative stress-responses in both healthy and diseased organism. The reduced sCLU protein levels in the RBCs membrane following cell exposure to various endogenous or exogenous stressors closely correlates to the levels of cellular senescence and redox imbalance markers, suggesting the usefulness of sCLU as a sensitive biomarker of senescence and cellular stress.	en
heal.journalName	PLoS ONE	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	false