Apolipoprotein J/clusterin in human erythrocytes is involved in the molecular process of defected material disposal during vesiculation

dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Τρουγκάκος, Ιωάννης Π.	el
dc.contributor.author	Παπασιδέρη, Ισιδώρα Σ.	el
dc.date.accessioned	2015-05-07T19:36:29Z
dc.date.available	2015-05-07T19:36:29Z
dc.date.issued	2015-05-07
dc.identifier.uri	http://hdl.handle.net/11400/9898
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0026033	en
dc.subject	απολιποπρωτεΐνη
dc.subject	ερυθροκύτταρα
dc.subject	μοριακή
dc.subject	ελαττωματικό υλικό
dc.subject	Κυστιδιοποίηση
dc.subject	apolipoprotein C
dc.subject	Erythrocytes
dc.subject	Molecular
dc.subject	Defected Material
dc.subject	Vesiculation
dc.title	Apolipoprotein J/clusterin in human erythrocytes is involved in the molecular process of defected material disposal during vesiculation	en
heal.type	journalArticle
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://lod.nal.usda.gov/136430
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85044747
heal.identifier.secondary	DOI: 10.1371/journal.pone.0026033
heal.language	en
heal.access	free
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2011-10-07
heal.bibliographicCitation	Antonelou, M.H., Kriebardis, A.G., Stamoulis, K.E., Trougakos, I.P. and Papassideri, I.S. (2011) Apolipoprotein J/clusterin in human erythrocytes is involved in the molecular process of defected material disposal during vesiculation. PLoS One. [Online] 6 (10), e26033. Available from: http://journals.plos.org [Accessed 07/05/2015]	en
heal.abstract	Background: We have showed that secretory Apolipoprotein J/Clusterin (sCLU) is down-regulated in senescent, stressed or diseased red blood cells (RBCs). It was hypothesized that sCLU loss relates to RBCs vesiculation, a mechanism that removes erythrocyte membrane patches containing defective or potentially harmful components. Methodology/Principal Findings: To investigate this issue we employed a combination of biochemical and microscopical approaches in freshly prepared RBCs or RBCs stored under standard blood bank conditions, an in vitro model system of cellular aging. We found that sCLU is effectively exocytosed in vivo during membrane vesiculation of freshly prepared RBCs. In support, the RBCs’ sCLU content was progressively reduced during RBCs ex vivo maturation and senescence under cold storage due to its selective exocytosis in membrane vesicles. A range of typical vesicular components, also involved in RBCs senescence, like Band 3, CD59, hemoglobin and carbonylated membrane proteins were found to physically interact with sCLU. Conclusions/Significance: The maturation of RBCs is associated with a progressive loss of sCLU. We propose that sCLU is functionally involved in the disposal of oxidized/defected material through RBCs vesiculation. This process most probably takes place through sCLU interaction with RBCs membrane proteins that are implicit vesicular components. Therefore, sCLU represents a pro-survival factor acting for the postponement of the untimely clearance of RBCs.	en
heal.journalName	PLoS ONE	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true