A simple reversed phase HPLC method suitable to study the interactions of alkylating agents with DNA is presented in this paper. DNA interaction is expressed as the % DNA peak size exclusion. The effects caused by the antitumor drugs melphalan, busulphan, and busulpan analogues on DNA were clearly observed through chromatographic data. The synthetic dimethanesulphonates of 2-tetradecyl-1,4-butanediol and 1,2-hexadecanediol were proved more potent than busulphan. Study of DNA interactions with melphalan, busulphan, and analogues using an HPLC method - ResearchGate. Available from: http://www.researchgate.net/publication/233043848_Study_of_DNA_interactions_with_melphalan_busulphan_and_analogues_using_an_HPLC_method [accessed May 9, 2015].