Invitro and invivo safety evaluation of the bacteriocin producer Streptococcusmacedonicus ACA-DC 198

dc.contributor.author	Μαραγκουδάκης, Πέτρος Α.	el
dc.contributor.author	Παπαδέλλη, Μαρίνα	el
dc.contributor.author	Γεωργαλάκη, Μαρίνα	el
dc.contributor.author	Παναγιωτοπούλου, Έφη Γ.	el
dc.contributor.author	Martinez-Gonzalez, Beatriz	en
dc.date.accessioned	2015-05-18T13:41:06Z
dc.date.available	2015-05-18T13:41:06Z
dc.date.issued	2015-05-18
dc.identifier.uri	http://hdl.handle.net/11400/10644
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S0168160509002657	en
dc.subject	Pathogenicity
dc.subject	Murine model
dc.subject	Παθογένεια
dc.subject	Μοντέλο ποντικού
dc.title	Invitro and invivo safety evaluation of the bacteriocin producer Streptococcusmacedonicus ACA-DC 198	en
heal.type	journalArticle
heal.classification	Food technology
heal.classification	Chemistry
heal.classification	Τεχνολογία τροφίμων
heal.classification	Χημεία
heal.classificationURI	http://skos.um.es/unescothes/C01577
heal.classificationURI	http://skos.um.es/unescothes/C00568
heal.classificationURI	N/A-Τεχνολογία τροφίμων
heal.classificationURI	N/A-Χημεία
heal.keywordURI	http://lod.nal.usda.gov/15187
heal.contributorName	Μεντής, Ανδρέας Φ.	el
heal.contributorName	Πετράκη, Καλλιόπη	el
heal.contributorName	Σγούρας, Διονύσιος Ν.	el
heal.contributorName	Τσακαλίδου, Έφη	el
heal.identifier.secondary	doi:10.1016/j.ijfoodmicro.2009.05.012
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Τεχνολογίας Τροφίμων.	el
heal.publicationDate	2009
heal.bibliographicCitation	Maragkoudakism P., Papadelli, M., Georgalaki, M., Panayotopoulou, E., Martinez-Gonzalez, B., et al. (July 2009). Invitro and invivo safety evaluation of the bacteriocin producer Streptococcusmacedonicus ACA-DC 198. International Journal of Food Microbiology. 133(1-2). pp. 141-147. Elsevier B.V: 2009. Available from: http://www.sciencedirect.com/science/article/pii/S0168160509002657 [Accessed 15/05/2009]	en
heal.abstract	Streptococcusmacedonicus ACA-DC 198, a bacteriocin producer isolated from Greek Kasseri cheese, was used in a series of invitro and invivo experiments in order to evaluate its pathogenic potential. The strain was examined invitro for haemolytic activity, antibiotic resistance and presence of pathogenicity genes encountered in Streptococcuspyogenes. Subsequently, the strain was orally administered to mice (8.9 log cfu daily), continuously over a period of 12 weeks, in order to ascertain the effects of its long term consumption on animal health and gastric inflammation. S.macedonicus ACA-DC 198 was found to be non-haemolytic and sensitive to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, streptomycin, tetracycline, and vancomycin, with the only resistance observed against kanamycin. PCR amplification and DNA–DNA hybridization did not reveal the presence of any of the S.pyogenes pathogenicity genes examined, namely emm, scpA, hasA, speB, smez2, speJ, sagAB, hylA, ska, speF, speG, slo, hylP2 and mga. In the mouse study, no detrimental effects were observed in the behaviour, general well being, weight gain and water consumption of the animals receiving S.macedonicus ACA-DC 198. Histologic analysis showed no evidence of inflammation in the stomach of the animals receiving S.macedonicus ACA-DC 198, while faecal microbiological analysis revealed that the strain retained its viability passing through the mouse gastrointestinal tract. Finally, no evidence of translocation to the liver, spleen and mesenteric lymph nodes was observed. In conclusion, none of the examined virulence determinants were detected in S.macedonicus ACA-DC 198 and its long term, high dosage oral administration did not appear to induce any pathogenic effect in mice.	en
heal.publisher	Elsevier B.V.	en
heal.journalName	International Journal of Food Microbiology	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true