High yield of secondary B-side electron transfer in mutant Rhodobacter capsulatus reaction centers

dc.contributor.author	Kressel, Lucas L.	el
dc.contributor.author	Faries, Kaitlyn M.	en
dc.contributor.author	Wander, Marc J.	en
dc.contributor.author	Ζόγκζας, Νικόλαος Π.	el
dc.contributor.author	Mejdrich, Rachel J.	el
dc.date.accessioned	2015-05-27T07:27:17Z
dc.date.available	2015-05-27T07:27:17Z
dc.date.issued	2015-05-27
dc.identifier.uri	http://hdl.handle.net/11400/11221
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S0005272814005532#	el
dc.subject	High throughput screening (Drug development)
dc.subject	Charge recombination
dc.subject	Χρέωση ανασυνδυασμού
dc.subject	Directed evolution
dc.subject	Κατευθυνόμενη εξέλιξη
dc.subject	Διαλογής υψηλής απόδοσης
dc.subject	Photosynthetic reaction center
dc.subject	Κέντρο αντίδρασης φωτοσυνθετικής
dc.subject	Transmembrane electron transfer
dc.subject	Διαμεμβρανική μεταφορά ηλεκτρονίων
dc.subject	Ultrafast spectroscopy
dc.subject	Υπερταχεία φασματοσκοπία
dc.title	High yield of secondary B-side electron transfer in mutant Rhodobacter capsulatus reaction centers	en
heal.type	journalArticle
heal.classification	Chemical technology
heal.classification	Food technology
heal.classification	Χημική τεχνολογία
heal.classification	Τεχνολογία τροφίμων
heal.classificationURI	http://skos.um.es/unescothes/C00565
heal.classificationURI	http://skos.um.es/unescothes/C01577
heal.classificationURI	N/A-Χημική τεχνολογία
heal.classificationURI	N/A-Τεχνολογία τροφίμων
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh2001002949
heal.contributorName	Hanson, Deborah K.	en
heal.contributorName	Holten, Dewey	en
heal.contributorName	Laible, Philip D.	en
heal.contributorName	Kirmaier, Christine	en
heal.identifier.secondary	ISSN: 00052728
heal.identifier.secondary	DOI: 10.1016/j.bbabio.2014.07.015
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων & Διατροφής. Τμήμα Τεχνολογίας Τροφίμων	el
heal.publicationDate	2014-11
heal.bibliographicCitation	Kressel, L. L., Faries, K. M, Wander, M. J., Zogzas, N. P., Mejdrich, R. J., et.al. (2014). High yield of secondary B-side electron transfer in mutant Rhodobacter capsulatus reaction centers. "Biochimica et Biophysica Acta - Bioenergetics", 1837(11), November 2014. pp 1892-1903. Available from: http://www.sciencedirect.com/science/article/pii/S0005272814005532. [Accessed 01/08/2014]	en
heal.abstract	From the crystal structures of reaction centers (RCs) from purple photosynthetic bacteria, two pathways for electron transfer (ET) are apparent but only one pathway (the A side) operates in the native protein-cofactor complex. Partial activation of the B-side pathway has unveiled the true inefficiencies of ET processes on that side in comparison to analogous reactions on the A side. Of significance are the relative rate constants for forward ET and the competing charge recombination reactions. On the B side, these rate constants are nearly equal for the secondary charge-separation step (ET from bacteriopheophytin to quinone), relegating the yield of this process to < 50%. Herein we report efforts to optimize this step. In surveying all possible residues at position 131 in the M subunit, we discovered that when glutamic acid replaces the native valine the efficiency of the secondary ET is nearly two-fold higher than in the wild-type RC. The positive effect of M131 Glu is likely due to formation of a hydrogen bond with the ring V keto group of the B-side bacteriopheophytin leading to stabilization of the charge-separated state involving this cofactor. This change slows charge recombination by roughly a factor of two and affords the improved yield of the desired forward ET to the B-side quinone terminal acceptor	en
heal.publisher	Elsevier B.V.	en
heal.journalName	Biochimica et Biophysica Acta - Bioenergetics	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true