Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery

dc.contributor.author	Τσουκάτος, Δημόκριτος Χ.	el
dc.contributor.author	Brochériou, Isabelle	fr
dc.contributor.author	Μούσσης, Βασίλειος	el
dc.contributor.author	Πανοπούλου, Χριστίνα Π.	el
dc.contributor.author	Χριστοφίδου, Έλενα Δ.	el
dc.date.accessioned	2015-05-28T05:45:29Z
dc.date.available	2015-05-28T05:45:29Z
dc.date.issued	2015-05-28
dc.identifier.uri	http://hdl.handle.net/11400/11316
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.jlr.org/content/49/10/2240	el
dc.subject	atherogenesis
dc.subject	Atherosclerosis
dc.subject	Lipoproteins
dc.subject	Macrophages
dc.subject	Αθηρογένεση
dc.subject	Αθηροσκλήρωση
dc.subject	Λιποπρωτεΐνες
dc.subject	Μακροφάγα
dc.subject	Oxidized LDL
dc.subject	Οξειδωμένη LDL
dc.subject	Smooth muscle cells
dc.subject	Κύτταρα λείου μυός
dc.title	Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery	en
heal.type	journalArticle
heal.classification	Medicine
heal.classification	Biochemistry
heal.classification	Ιατρική
heal.classification	Βιοχημεία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh00006614
heal.classificationURI	http://skos.um.es/unescothes/C00386
heal.classificationURI	N/A-Ιατρική
heal.classificationURI	N/A-Βιοχημεία
heal.keywordURI	http://lod.nal.usda.gov/13589
heal.keywordURI	http://skos.um.es/unesco6/320702
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85077309
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85079451
heal.contributorName	Κουσίσης, Σταμάτης	el
heal.contributorName	Σισμανίδης, Σωκράτης	el
heal.contributorName	Ninio, Ewa	en
heal.contributorName	Συμινελάκης, Σταύρος Ν.	el
heal.identifier.secondary	ISSN: 00222275
heal.identifier.secondary	DOI: 10.1194/jlr.M800188-JLR200
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων & Διατροφής. Τμήμα Τεχνολογίας Τροφίμων	el
heal.publicationDate	2008-10
heal.bibliographicCitation	Tsoukatos, D. C., Brochériou, I., Moussis, V., PanopoUlou C. P., Christofidou, E. D., et.al. (2008). Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery. "Journal of Lipid Research", 49(10), October 2008. pp 2240-2249. Available from: http://www.jlr.org/content/49/10/2240. [Accessed 27/06/2008]	en
heal.abstract	Platelet-activating factor (PAF), the potent phospholipid mediator of inflammation, is involved in atherosclerosis. Platelet-activating factor-acetylhydrolase (PAF-AH), the enzyme that inactivates PAF bioactivity, possesses both acetylhydrolase and transacetylase activities. In the present study, we measured acetylhydrolase and transacetylase activities in human atherogenic aorta and nonatherogenic mammary arteries. Immunohistochemistry analysis showed PAF-AH expression in the intima and the media of the aorta and in the media of mammary arteries. Acetylhydrolase and transacetylase activities were (mean ± SE, n = 38): acetylhydrolase of aorta, 2.8 ± 0.5 pmol/ min/mg of tissue; transacetylase of aorta, 3.3 ± 0.7 pmol/min/mg of tissue; acetylhydrolase of mammary artery, 1.4 ± 0.3 pmol/min/ mg of tissue (P< 0.004 as compared with acetylhydrolase of aorta); transacetylase of mammary artery, 0.8 ± 0.2 pmol/min/mg of tissue (P < 0.03 as compared with acetylhydrolase of mammary artery). Lyso-PAF accumulation and an increase in PAF bioactivity were observed in the aorta of some patients. Reverse-phase HPLC and electrospray ionization mass spectrometry analysis revealed that 1-O-hexadecyl-2 acetyl-sn glycero-3-phosphocholine accounted for 60% of the PAF bioactivity and 1-O-hexadecyl-2-butanoyl-sn-glycerol-3-phosphocholine for 40% of the PAF bioactivity. The nonatherogenic properties of mammary arteries may in part be due to low PAF formation regulated by PAF-AH activity. In atherogenic aortas, an imbalance between PAF-AH and transacetylase activity, as well as lyso-PAF accumulation, may lead to unregulated PAF formation and to progression of atherosclerosis	en
heal.publisher	American Society for Biochemistry and Molecular Biology	en
heal.journalName	Journal of Lipid Research	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true