On the combined application of Iatroscan-TLC-FID and GC-FID to identify total, neutral and polar lipids and their fatty acids extracted from foods

dc.contributor.author	Σινάνογλου, Βασιλεία	el
dc.contributor.author	Στρατή, Ειρήνη	el
dc.contributor.author	Μπρατάκος, Σωτήριος	el
dc.contributor.author	Προεστός, Χαράλαμπος	el
dc.contributor.author	Ζουμπουλάκης, Παναγιώτης	el
dc.date.accessioned	2015-05-29T17:57:14Z
dc.date.available	2015-05-29T17:57:14Z
dc.date.issued	2015-05-29
dc.identifier.uri	http://hdl.handle.net/11400/11843
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.hindawi.com	en
dc.source	http://www.hindawi.com/journals/isrn/2013/859024/	en
dc.subject	Λιπίδια
dc.subject	Λιπαρά οξέα
dc.subject	Φυτικές ίνες
dc.subject	Ζωικά λίπη
dc.title	On the combined application of Iatroscan-TLC-FID and GC-FID to identify total, neutral and polar lipids and their fatty acids extracted from foods	en
heal.type	journalArticle
heal.classification	Food
heal.classification	Food technology
heal.classification	Τροφή
heal.classification	Τεχνολογία τροφίμων
heal.classificationURI	http://id.loc.gov/authorities/names/n00009376
heal.classificationURI	http://skos.um.es/unescothes/C01577
heal.classificationURI	N/A-Τροφή
heal.classificationURI	N/A-Τεχνολογία τροφίμων
heal.contributorName	Μηνιάδου - Μεϊμάρογλου, Σοφία	el
heal.identifier.secondary	DOI: 10.1155/2013/859024
heal.language	en
heal.access	free
heal.publicationDate	2012-12-11
heal.bibliographicCitation	Sinanoglou, V., Strati, I., Bratakos, S., Proestos, C., Zoumpoulakis, P. et al. (2012) On the combined application of Iatroscan-TLC-FID and GC-FID to identify total, neutral and polar lipids and their fatty acids extracted from foods. "ISRN Chromatography", 2013	en
heal.abstract	An efficient separation and quantification of the individual neutral and polar lipid classes and their constituent fatty acids was achieved by the combination of two different detection techniques: Iatroscan TLC-FID and GC-FID. The solvent composition and ratio of development system, the sample size, the fidelity, and precision were tested in order to estimate the effectiveness of separation of individual neutral and polar lipid classes and the quantitative reproducibility of the Iatroscan TLC-FID technique. GC-FID method, with a high-quality capillary column, allowed sensitive and reproducible fatty acid qualitative and quantitative analyses, separation of fatty acid structural isomers (e.g., n-C16:0, iso-C16:0 and anteiso-C16:0), positional isomers (e.g., C18:1ω-9 and C18:1ω-7), geometrical isomers (cis-trans), and homologues (e.g., C16:0, C17:0, C18:0, etc.) in standards and complex lipid samples. Seventeen (17) lipid classes and fifty-two (52) saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids were identified and quantified, respectively, in samples of standard lipid and fatty acid mixtures, simulating the composition of natural lipids and their fatty acid methyl esters in common foods. The wide number of applications establishes this combination of Iatroscan TLC-FID and GC-FID methods as a powerful tool for lipid class and fatty acid analysis of any fat origin.	el
heal.publisher	Hindawi Publishing Corporation	en
heal.journalName	ISRN Chromatography	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true