Separation and quantification of marine lipids using Iatroscan thin-layer chromatographic-flame ionisation detection analysis

dc.contributor.author	Παπαδάς, Γιώργος	el
dc.contributor.author	Σινάνογλου, Βασιλεία	el
dc.contributor.author	Μηνιάδου - Μεϊμάρογλου, Σοφία	el
dc.date.accessioned	2015-06-02T17:30:11Z
dc.date.issued	2015-06-02
dc.identifier.uri	http://hdl.handle.net/11400/14881
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.eurofedlipid.org/	en
dc.subject	Λιπίδια
dc.subject	Ιονισμός
dc.subject	Ψάρια
dc.subject	Fish
dc.subject	Χρωμογραφική ανάλυση
dc.title	Separation and quantification of marine lipids using Iatroscan thin-layer chromatographic-flame ionisation detection analysis	en
heal.type	conferenceItem
heal.classification	Food
heal.classification	Food technology
heal.classification	Τροφή
heal.classification	Τεχνολογία τροφίμων
heal.classificationURI	http://id.loc.gov/authorities/names/n00009376
heal.classificationURI	http://skos.um.es/unescothes/C01577
heal.classificationURI	N/A-Τροφή
heal.classificationURI	N/A-Τεχνολογία τροφίμων
heal.dateAvailable	10000-01-01
heal.language	en
heal.access	forever
heal.publicationDate	2008-09-07
heal.bibliographicCitation	Papadas, G., Sinanoglou, V. and Miniadis-Meimaroglou, S. (2008) Separation and quantification of marine lipids using Iatroscan thin-layer chromatographic–flame ionisation detection analysis. In 6th Euro Fed Lipid Congress: Oils, Fats and Lipids in the 3rd Millennium. 7th to 10th September 2008. Athens	en
heal.abstract	A method for separation and quantification of neutral lipid and phospholipid classes was developed using Iatroscan analysis, which combines thin layer chromatography (TLC) and flame ionisation detection (FID) techniques. Neutral lipid classes were developed and separated in several solvent systems with hexane-diethyl ether-formic acid (H:D:F). Phospholipid classes were developed and separated in several solvent systems with chloroform-methanol-water (C:M:W), chloroform-methanol-water-formic acid (C:M:W:F) and chloroform-methanol-water-ammonia (C:M:W:A). Finally, neutral lipid and phospholipid classes were developed and separated by several multiple developments using 2 or 3 different development solvent systems of decreasing polarity. The bath temperature was 25oC. The measurement conditions were hydrogen flow-rate 160 mL/min, air flow-rate 2L/min and scan speed 30s/scan. Even a slight difference in solvent proportion or composition of development solution influenced the chromatogram pattern as well as the individual lipid classes’ fractionation. Thirteen lipid classes were identified and quantified in samples of standard lipid mixtures and marine lipids using the proposed development solvent systems. Iatroscan analysis is a novel technique that is rapid, sensitive, easy to handle and exhibits a high reproducibility.	en
heal.fullTextAvailability	true
heal.conferenceName	6th Euro Fed Lipid Congress: Oils, Fats and Lipids in the 3rd Millennium	en
heal.conferenceItemType	poster