Effects of pre-storage leukoreduction on stored red blood cells signaling

dc.contributor.author	Αντωνέλου, Μαριάννα	el
dc.contributor.author	Τζουνάκας, Βασίλης Λ.	el
dc.contributor.author	Βελέντζας, Αθανάσιος	el
dc.contributor.author	Σταμούλης, Κωνσταντίνος	el
dc.contributor.author	Κριεμπάρδης, Αναστάσιος Γ.	el
dc.date.accessioned	2015-05-07T19:52:22Z
dc.date.available	2015-05-07T19:52:22Z
dc.date.issued	2015-05-07
dc.identifier.uri	http://hdl.handle.net/11400/9899
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S1874391912005295	en
dc.subject	Αποθήκευση
dc.subject	ερυθροκύτταρα
dc.subject	γήρανση
dc.subject	Λευκαφαίρεση προ-αποθήκευσης
dc.subject	Μεμβράνη πρωτέωμα
dc.subject	Οξειδωτικό στρες
dc.subject	Storage
dc.subject	Erythrocytes
dc.subject	Pre-storage leukoreduction
dc.subject	Membrane proteome
dc.subject	Oxidative stress
dc.title	Effects of pre-storage leukoreduction on stored red blood cells signaling	en
heal.type	journalArticle
heal.secondaryTitle	a time-course evaluation from shape to proteome	en
heal.classification	Βιοφυσική
heal.classification	Κυτταρολογία
heal.classification	Biophysics
heal.classification	Cell biology
heal.classificationURI	N/A-Βιοφυσική
heal.classificationURI	N/A-Κυτταρολογία
heal.classificationURI	http://id.loc.gov/authorities/subjects/sh85014253
heal.classificationURI	http://skos.um.es/unescothes/C00536
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh00008427
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85044747
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh2003008365
heal.contributorName	Παπασιδέρη, Ισιδώρα Σ.	el
heal.identifier.secondary	DOI: 10.1016/j.jprot.2012.06.032
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Επαγγελμάτων Υγείας και Πρόνοιας. Τμήμα Ιατρικών Εργαστηρίων	el
heal.publicationDate	2012-12-05
heal.bibliographicCitation	Antonelou, M.H., Tzounakas, V.L., Velentzas, A.D., Stamoulis, K.E., Kriebardis, A.G. et al. (2012) Effects of pre-storage leukoreduction on stored red blood cells signaling: A time-course evaluation from shape to proteome. Journal of Proteomics. [Online] 76, pp.220–238. Available from: http://www.sciencedirect.com [Accessed 07/05/2015]	en
heal.abstract	The introduction of pre-storage leukoreduction in the preparation of standard RBCs intended for transfusion provided significant improvement in the quality of labile products and their post transfusion viability and effects, although the literature data are controversial. To elucidate the issue of the probable leukoreduction effects on RBCs storage lesion, we evaluated various storage quality measures in RBCs stored in either leukoreduced (L) or non-leukoreduced (N) units, with emphasis to senescence and oxidative stress associated modifications. Our data suggest that the residual leukocytes/platelets of the labile products represent a stressful storage factor, countering the structural and functional integrity of stored RBCs. Hemolysis, irreversible echinocytosis, microvesiculation, removal signaling, ROS/calcium accumulation, band 3-related senescence modifications, membrane proteome stress biomarkers as well as emergence of a senescence phenotype in young RBCs that is disproportionate to their age, are all encountered more or mostly in N-RBCs compared to the L-RBCs, either for a part or for the whole of the storage period. The partial, yet significant, alleviation of so many storage-related manifestations in the L-RBCs compared to the N-RBCs, is presented for the first time and provides a rational mechanistic interpretation of the improved storage quality and transfusions observed by the introduction of pre-storage leukoreduction. This article is part of a Special Issue entitled: Integrated omics.	en
heal.publisher	Elsevier	en
heal.journalName	Journal of Proteomics	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true