Total polar lipid analysis in marine shrimp P.kerathurus by HR NMR spectroscopy

dc.contributor.author	Κόρα, Λιντίτα	el
dc.contributor.author	Ζουμπουλάκης, Παναγιώτης	el
dc.contributor.author	Σινάνογλου, Βασιλεία	el
dc.contributor.author	Μηνιάδου - Μεϊμάρογλου, Σοφία	el
dc.date.accessioned	2015-06-03T16:17:19Z
dc.date.issued	2015-06-03
dc.identifier.uri	http://hdl.handle.net/11400/14972
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.eurofedlipid.org	en
dc.subject	Πολικά λιπίδια
dc.subject	Γαρίδες
dc.subject	Λιπαρά οξέα
dc.subject	Fatty acids
dc.title	Total polar lipid analysis in marine shrimp P.kerathurus by HR NMR spectroscopy	en
heal.type	conferenceItem
heal.secondaryTitle	study of carotenoids using HPLC	en
heal.classification	Food
heal.classification	Food technology
heal.classification	Τροφή
heal.classification	Τεχνολογία τροφίμων
heal.classificationURI	http://id.loc.gov/authorities/names/n00009376
heal.classificationURI	http://skos.um.es/unescothes/C01577
heal.classificationURI	N/A-Τροφή
heal.classificationURI	N/A-Τεχνολογία τροφίμων
heal.dateAvailable	10000-01-01
heal.language	en
heal.access	forever
heal.publicationDate	2011-09-18
heal.bibliographicCitation	Lindita, K., Zoumpoulakis, P., Sinanoglou, V. and Miniadou-Meimaroglou, S. (2011) Total polar lipid analysis in marine shrimp P.kerathurus by HR NMR spectroscopy. Study of carotenoids using HPLC. In 9th Eurofed Lipids Congress - Oils, Fats and Lipids for a Healthy and Sustainable World. 18th to 21st September 2011. Rotterdam	en
heal.abstract	High Resolution NMR spectroscopy offers a fast and sensitive method for qualitative and molecular structure analysis of polar lipids (PhL) and their main compounds (PC and PE). Lipids of marine shrimp Penaeus kerathurus were extracted according to the method of Bligh and Dyer. SPE technique was implemented to separate polar from total lipids. High resolution NMR spectroscopy has been applied for the assessment of lipid classes in marine phospholipids. More specifically, 1D 1H and 2D COSY and HSQC techniques were implemented in order to elucidate the characteristic peaks of phospholipids in the polar extracts. Furthermore, 31P NMR and spiking experiments using reference standard polar lipids (PC, PE, SPH, LPC) differentiated phospholipids and assigned several peaks to specific lipid classes. In conclusion, marine shrimp Penaeus kerathurus contains in high concentration PC and PE phospholipids and less SPH and LPC. Low inensity deshielded peaks (around 7-8 ppm) were attributed to unknown compounds for further investigations. Furthermore, carotenoid pigments are responsible for the more or less marked colouration of marine shrimps. The carotenoid profile and content of Penaeus kerathurus muscle and cephalothorax lipids, were evaluated using high-performance liquid chromatography (HPLC). The major carotenoids found were astaxanthin as well as its mono- and di- esters.	en
heal.fullTextAvailability	true
heal.conferenceName	9th Eurofed Lipids Congress - Oils, Fats and Lipids for a Healthy and Sustainable World	en
heal.conferenceItemType	poster