Determination of 1-aminocyclopropane-1-carboxylic acid and its structural analogue by liquid chromatography and ion spray tandem mass spectrometry

dc.contributor.author	Πετρίτης, Κωνσταντίνος Ν.	el
dc.contributor.author	Ντουρτόγλου, Βασίλειος Γ.	el
dc.contributor.author	Elfakir, Claire	en
dc.contributor.author	Dreux, Michel	el
dc.date.accessioned	2015-05-23T07:58:05Z
dc.date.available	2015-05-23T07:58:05Z
dc.date.issued	2015-05-23
dc.identifier.uri	http://hdl.handle.net/11400/10940
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S0021967300007573	el
dc.subject	Amino acids
dc.subject	Carboxylic acids
dc.subject	food analysis
dc.subject	Αμινοξέα
dc.subject	Aminocyclopropanecarboxylic acid
dc.subject	Αμινοκυκλοπροπανοκαρβοξυλικό οξύ
dc.subject	Apples
dc.subject	Μήλα
dc.subject	Καρβοξυλικά οξέα
dc.subject	Cyclopropanedicarboxylic acid
dc.subject	Κυκλοπροπανοδικαρβοξυλικό οξύ
dc.subject	Ανάλυση τροφίμων
dc.title	Determination of 1-aminocyclopropane-1-carboxylic acid and its structural analogue by liquid chromatography and ion spray tandem mass spectrometry	en
heal.type	journalArticle
heal.generalDescription	Δημοσιευμένο και στο: 6th International Symposium on Hyphenated Techniques in Chromatography	el
heal.classification	Food technology
heal.classification	Biochemistry
heal.classification	Τεχνολογία τροφίμων
heal.classification	Βιοχημεία
heal.classificationURI	http://skos.um.es/unescothes/C01577
heal.classificationURI	http://skos.um.es/unescothes/C00386
heal.classificationURI	N/A-Τεχνολογία τροφίμων
heal.classificationURI	N/A-Βιοχημεία
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85004486
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85020144
heal.keywordURI	http://lod.nal.usda.gov/178
heal.identifier.secondary	doi:10.1016/S0021-9673(00)00757-3
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών	el
heal.publicationDate	2000-10
heal.bibliographicCitation	Petritis, K., Dourtoglou, V. G. Elfakir, C. and Dreux, M. (2000). Inhibition of apple 1-aminocyclopropane-1-carboxylic acid oxidase, by cyclopropane-1,1-dicarboxylic acid and trans-2-phenylcyclopropane-1-carboxylic acid. 6th International Symposium on Hyphenated Techniques in Chromatography "Journal of Chromatography A", vol. 896, October 2000. pp. 335–341. Available from: http://www.sciencedirect.com/science/article/pii/S0021967300007573. [Accessed 18/10/2000]	en
heal.abstract	Liquid chromatography coupled to ion spray tandem mass spectrometry was developed as a method for the simultaneous analysis of the amino acid 1-aminocyclopropane-1-carboxylic acid (ACC) and its structural analogue, cyclopropane-1,1-dicarboxylic acid (CDA). ACC and CDA fragmentation as well as optimization of MS parameters were investigated in positive ion mode. In selective reaction monitoring mode the protonated molecule [M+H]+ was selected as parent ion for both ACC and CDA, while the immonium ion from ACC and the [M+H-H2O]+ ion from CDA were selected, respectively, as product ions. In spite of the high selectivity of MS/MS among the 20 protein amino acids potentially present with ACC and CDA in the plant material analyzed, Glu and Thr can interfere with the signal of ACC. As a result, their chromatographic separation is necessary. This was achieved in less than 4 min by ion-pair reversed-phase chromatography with nonafluoropentanoic acid as ion-pair reagent. A linear response within a concentration range of 1-5 mg l-1 was observed for this LC method and the detection limit was found to be 20 pmol for ACC and 150 pmol for CDA (using a 20-μl loop). This methodology was successfully applied to the detection of ACC in apple tissue.	en
heal.publisher	Elsevier Science B.V.	en
heal.journalName	Journal of Chromatography A	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true