A defence-related Olea europaea β-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent

dc.contributor.author	Κουδούνας, Κωνσταντίνος	el
dc.contributor.author	Μπανίλας, Γεώργιος Π.	el
dc.contributor.author	Μιχαηλίδης, Χρήστος	el
dc.contributor.author	Δημολιού, Αικατερίνη Δ.	el
dc.contributor.author	Ρήγας, Σταμάτης	el
dc.date.accessioned	2015-05-23T13:09:23Z
dc.date.available	2015-05-23T13:09:23Z
dc.date.issued	2015-05-23
dc.identifier.uri	http://hdl.handle.net/11400/10978
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://jxb.oxfordjournals.org/content/66/7/2093	el
dc.subject	oleuropein
dc.subject	Heterologous expression
dc.subject	Ετερόλογη έκφραση
dc.subject	Ελευρωπαΐνη
dc.subject	Olive development
dc.subject	Ανάπτυξη ελιάς
dc.subject	Plant defence
dc.subject	Άμυνα των φυτών
dc.subject	Protein cross-linking
dc.subject	β-Glucosidase
dc.title	A defence-related Olea europaea β-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent	en
heal.type	journalArticle
heal.classification	Agriculture
heal.classification	Biology
heal.classification	Γεωργία
heal.classification	Βιολογία
heal.classificationURI	http://skos.um.es/unescothes/C00112
heal.classificationURI	http://skos.um.es/unescothes/C00399
heal.classificationURI	N/A-Γεωργία
heal.classificationURI	N/A-Βιολογία
heal.keywordURI	http://lod.nal.usda.gov/55790
heal.contributorName	Χατζόπουλος, Πολυδεύκης	el
heal.identifier.secondary	doi: 10.1093/jxb/erv002
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών	el
heal.publicationDate	2011-04-01
heal.bibliographicCitation	Koudounas, K., Banilas, G. P., Michaelidis, C., Demoliou, C. D., Rigas, S, et.al. (2015). A defence-related Olea europaea β-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent. "Journal of Experimental Botany", 66(7), 1 April 2015. pp 2093-2106. Available from: http://jxb.oxfordjournals.org/content/66/7/2093. [Accessed 19/02/2015]	en
heal.abstract	Oleuropein, the major secoiridoid compound in olive, is involved in a sophisticated two-component defence system comprising a β-glucosidase enzyme that activates oleuropein into a toxic glutaraldehyde-like structure. Although oleuropein deglycosylation studies have been monitored extensively, an oleuropein β-glucosidase gene has not been characterized as yet. Here, we report the isolation of OeGLU cDNA from olive encoding a β-glucosidase belonging to the defence-related group of terpenoid-specific glucosidases. In planta recombinant protein expression assays showed that OeGLU deglycosylated and activated oleuropein into a strong protein cross-linker. Homology and docking modelling predicted that OeGLU has a characteristic (β/α)8 TIM barrel conformation and a typical construction of a pocket-shaped substrate recognition domain composed of conserved amino acids supporting the β-glucosidase activity and non-conserved residues associated with aglycon specificity. Transcriptional analysis in various olive organs revealed that the gene was developmentally regulated, with its transcript levels coinciding well with the spatiotemporal patterns of oleuropein degradation and aglycon accumulation in drupes. OeGLU upregulation in young organs reflects its prominent role in oleuropein-mediated defence system. High gene expression during drupe maturation implies an additional role in olive secondary metabolism, through the degradation of oleuropein and reutilization of hydrolysis products	en
heal.publisher	Oxford University Press on behalf of the Society for Experimental Biology	en
heal.journalName	Journal of Experimental Botany	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true