Genome-wide analysis of the heat shock protein 90 gene family in grapevine (Vitis vinifera L.)

dc.contributor.author	Μπανίλας, Γεώργιος Π.	el
dc.contributor.author	Κόρκας, Ηλίας	el
dc.contributor.author	Εγγλέζος, Βασίλειος	el
dc.contributor.author	Νησιώτου, Ασπασία Α.	el
dc.contributor.author	Χατζόπουλος, Πολυδεύκης	el
dc.date.accessioned	2015-05-24T06:46:13Z
dc.date.available	2015-05-24T06:46:13Z
dc.date.issued	2015-05-24
dc.identifier.uri	http://hdl.handle.net/11400/11021
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0238.2011.00166.x/abstract	el
dc.subject	Droughts
dc.subject	Gene expression
dc.subject	Ξηρασία
dc.subject	Γονιδιακή έκφραση
dc.subject	Heat shock
dc.subject	Θερμικό σοκ
dc.subject	Hsp90
dc.subject	Molecular response
dc.subject	Μοριακή ανταπόκριση
dc.subject	Stress protein
dc.subject	Πρωτεΐνη του στρες
dc.title	Genome-wide analysis of the heat shock protein 90 gene family in grapevine (Vitis vinifera L.)	en
heal.type	journalArticle
heal.classification	Agriculture
heal.classification	Biology
heal.classification	Γεωργία
heal.classification	Βιολογία
heal.classificationURI	http://skos.um.es/unescothes/C00112
heal.classificationURI	http://skos.um.es/unescothes/C00399
heal.classificationURI	N/A-Γεωργία
heal.classificationURI	N/A-Βιολογία
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85039663
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85053734
heal.identifier.secondary	DOI: 10.1111/j.1755-0238.2011.00166.x
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών	el
heal.publicationDate	2012-02
heal.bibliographicCitation	Banilas, G., Korkas, E., Englezos, V., Nisiotou, A. A. and Hatzopoulos, P. (2012). Genome-wide analysis of the heat shock protein 90 gene family in grapevine (Vitis vinifera L.). "Australian Journal of Grape and Wine Research", 18(1), February 2012. pp 29-38. Available from: http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0238.2011.00166.x/abstract. [Accessed 19/10/2011]	en
heal.abstract	Background and Aims: Heat shock protein 90 (Hsp90) proteins constitute an important gene family of molecular chaperons. High-temperature stress, which is often combined with drought stress, may exert major constraints to grapevine growth and development. The aim of this study was to characterise the Vitisvinifera Hsp90 gene family. Methods and Results: Using the complete grapevine genome sequences, four cytoplasmic and three organelle-specific V. vinifera Hsp90 (VvHsp90) proteins were identified. Phylogenetic analysis revealed that they share high sequence similarity with their Arabidopsis counterparts, while the cytosolic isoforms are clustered into two distinct groups (VvHsp90.1 and VvHsp90.2). Transcriptional analysis showed that a representative gene from the first group (VvHsp90.1a), in contrast to VvHsp90.2a, is induced by heat shock in all vegetative tissues/organs tested. Interestingly, it was also expressed in tendrils in the absence of stress. The severity and duration of heat stress influenced in a complex manner the expression profile of VvHsp90.1a, while the other VvHsp90s tested were rather constitutively regulated. However, the endoplasmic reticulum-specific VvHsp90.7 was mildly and transiently induced by a relatively prolonged heat stress. Combined drought with heat stress resulted in a delay in VvHsp90.1a induction. Conclusions: Gene structure organisation and expression characteristics of VvHsp90s resemble those of their Arabidopsis orthologs, although species-specific differences also exist. Differential regulation of genes suggests functional diversification among isoforms. Significance of the Study: This is the first report on the characterisation of Hsp90s in grapevine. The present study contributes to a deeper understanding of the complex molecular responses of grapevine to stress.	en
heal.publisher	Australian Society of Viticulture and Oenology Inc	en
heal.journalName	Australian Journal of Grape and Wine Research	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true