The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1

dc.contributor.author	Μπανίλας, Γεώργιος Π.	el
dc.contributor.author	Καράμπελας, Μιχαήλ	el
dc.contributor.author	Μακαρίτη, Ιφιγένεια Π.	el
dc.contributor.author	Κούρτη, Άννα Τ.	el
dc.contributor.author	Χατζόπουλος, Πολυδεύκης	el
dc.date.accessioned	2015-05-24T07:13:33Z
dc.date.available	2015-05-24T07:13:33Z
dc.date.issued	2015-05-24
dc.identifier.uri	http://hdl.handle.net/11400/11023
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://jxb.oxfordjournals.org/content/62/2/521	el
dc.subject	diacylglycerol acyltransferase
dc.subject	Gene expression
dc.subject	In situ hybridization
dc.subject	triacylglycerols
dc.subject	Διακυλγλυκερίνη ακυλτρανσφεράση
dc.subject	Γονιδιακή έκφραση
dc.subject	In situ υβριδοποίηση
dc.subject	Olive development
dc.subject	Ανάπτυξη ελιάς
dc.subject	Τριγλυκερίδια
dc.title	The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1	en
heal.type	journalArticle
heal.classification	Agriculture
heal.classification	Biology
heal.classification	Γεωργία
heal.classification	Βιολογία
heal.classificationURI	http://skos.um.es/unescothes/C00112
heal.classificationURI	http://skos.um.es/unescothes/C00399
heal.classificationURI	N/A-Γεωργία
heal.classificationURI	N/A-Βιολογία
heal.keywordURI	http://lod.nal.usda.gov/68103
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85053734
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh90000762
heal.keywordURI	http://lod.nal.usda.gov/3995
heal.identifier.secondary	doi: 10.1093/jxb/erq286
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών	el
heal.publicationDate	2011-01
heal.bibliographicCitation	Banilas, G. Karampelias, M., Makariti, I. P., Kourti, A. T. and Hatzopoulos, P. (2011). The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1. "Journal of Experimental Botany", 62(2), January 2011. pp 521-532. Available from: http://jxb.oxfordjournals.org/content/62/2/521. [Accessed 24/09/2010]	en
heal.abstract	Diacylglycerol acyltransferases (DGATs) catalyse the final step of the triacylglycerol (TAG) biosynthesis of the Kennedy pathway. Two major gene families have been shown to encode DGATs, DGAT1 (type-1) and DGAT2 (type-2). Both genes encode membrane-bound proteins, with no sequence homology to each other. In this study, the molecular cloning and characterization of a type-2 DGAT cDNA from olive is presented. Southern blot analysis showed that OeDGAT2 is represented by a single copy in the olive genome. Comparative transcriptional analysis revealed that DGAT1 and DGAT2 are developmentally regulated and share an overall overlapping but distinct transcription pattern in various tissues during vegetative growth. DGAT2 is highly expressed in mature or senescing olive tissues. In flowers, the expression of DGAT1 was almost undetectable, while DGAT2 transcripts accumulated at the later stages of both anther and ovary development. Differential gene regulation was also detected in the seed and mesocarp, two drupe compartments that largely differ in their functional roles and mode of lipid accumulation. DGAT1 appears to contribute for most of the TAG deposition in seeds, whereas, in the mesocarp, both DGAT1 and DGAT2 share an overlapping expression pattern. During the last stages of mesocarp growth, when TAGs are still accumulating, strong up-regulation of DGAT2 but a marked decline of DGAT1 transcript levels were detected. The present results show overlapping gene expression for olive DGATs during mesocarp growth, with a more prominent implication of DGAT2 in floral bud development and fruit ripening	en
heal.journalName	Journal of Experimental Botany	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true