Spatial and temporal expressions of two distinct oleate desaturases from olive (Olea europaea L.)

dc.contributor.author	Μπανίλας, Γεώργιος Π.	el
dc.contributor.author	Μορέσσης, Αναστάσιος	el
dc.contributor.author	Νικολουδάκης, Νικόλαος	el
dc.contributor.author	Χατζόπουλος, Πολυδεύκης	el
dc.date.accessioned	2015-05-24T08:37:41Z
dc.date.available	2015-05-24T08:37:41Z
dc.date.issued	2015-05-24
dc.identifier.uri	http://hdl.handle.net/11400/11026
dc.rights	Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.source	http://www.sciencedirect.com/science/article/pii/S0168945204004261#	el
dc.subject	Olive
dc.subject	FAD2
dc.subject	FAD6
dc.subject	Fatty acid desaturase
dc.subject	Λιπαρού οξέος δεσατουράση
dc.subject	Ελιά
dc.subject	Spatial expression
dc.subject	Χωρική έκφραση
dc.subject	Temporal expression
dc.subject	Χρονική έκφραση
dc.title	Spatial and temporal expressions of two distinct oleate desaturases from olive (Olea europaea L.)	en
heal.type	journalArticle
heal.classification	Agriculture
heal.classification	Biology
heal.classification	Γεωργία
heal.classification	Βιολογία
heal.classificationURI	http://skos.um.es/unescothes/C00112
heal.classificationURI	http://skos.um.es/unescothes/C00399
heal.classificationURI	N/A-Γεωργία
heal.classificationURI	N/A-Βιολογία
heal.keywordURI	http://id.loc.gov/authorities/subjects/sh85094612
heal.identifier.secondary	doi:10.1016/j.plantsci.2004.09.026
heal.language	en
heal.access	campus
heal.recordProvider	Τεχνολογικό Εκπαιδευτικό Ίδρυμα Αθήνας. Σχολή Τεχνολογίας Τροφίμων και Διατροφής. Τμήμα Οινολογίας και Τεχνολογίας Ποτών	el
heal.publicationDate	2005-02
heal.bibliographicCitation	Banilas, G., Moressis, A., Nikoloudakis, N. and Hatzopoulos, P. (2005). Spatial and temporal expressions of two distinct oleate desaturases from olive (Olea europaea L.). "Plant Science", 168(2), February. pp 547-555. Available from: http://www.sciencedirect.com/science/article/pii/S0168945204004261#. [Accessed 19/10/2004]	en
heal.abstract	Two cDNAs, OeFAD2 and OeFAD6, encoding ω-6 fatty acid desaturases, the key enzymes for the conversion of oleic into linoleic acid, were isolated from olive. OeFAD2 contains a C-terminal ER retrieval motif, whereas OeFAD6 possess a putative N-terminal plastidial signal peptide. The deduced amino acid sequence of OeFAD2 showed higher similarities to other plant microsomal ω-6 desasurases than to OeFAD6 or plastidial orthologues, and vice versa. Southern analysis indicated that the OeFAD2 gene is represented by one or two copies and OeFAD6 by a single copy gene. Expression analysis by RT-PCR showed that both genes are expressed in all tissues of olive tree tested, but higher levels of mRNA accumulation were detected in reproductive organs and cells that proliferate rapidly or store lipids. The two genes exhibited distinct patterns of mRNA accumulation during olive drupe growth. OeFAD2 was constitutively expressed, with maximum transcript accumulation in mesocarps, whereas OeFAD6 was developmentally regulated in both endosperms and embryos. The expression patterns observed reflect the discrete roles of these genes in membrane synthesis for cell division, thylakoid development, and lipid storage or in the biosynthetic pathway for the production of signaling molecules that influence plant development or defense.	en
heal.publisher	Elsevier Ireland Ltd	en
heal.journalName	Plant Science	en
heal.journalType	peer-reviewed
heal.fullTextAvailability	true